mtnK

5-methylthioribose kinase

Locus: BSU_13560

Isoelectric point: 4.86

Molecular weight: 45.19 kDa

Protein length: 399 aa

Gene length: 1200 bp

Function: methionine salvage

Product: 5-methylthioribose kinase

Essential: no

E.C.: 2.7.1.100

Synonyms: ykrT

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of methionine/ S-adenosylmethionine
• Groups of genes → Membrane proteins
• Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

Gene

Coordinates: 1,423,241 → 1,424,440

The protein

Catalyzed reaction/ biological activity

• ATP + S-methyl-5-thio-D-ribose --> ADP + H⁺ + S-methyl-5-thio-α-D-ribose 1-phosphate (according to UniProt)

Protein family

• methylthioribose kinase family (single member, according to UniProt)

Modification

• phosphorylated on Arg-80 OR Arg-82 and Arg-365 PubMed

Structure

• 2PUP

Localization

• membrane associated PubMed

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed
• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: mtnK-mtnA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • S-box: termination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box

  Regulation

  • induced by methionine starvation (S-box) PubMed
  • the S-box RNA is degraded by RNase Y PubMed

Biological materials

Mutant

• MGNA-A783 (ykrT::erm), available at the NBRP B. subtilis, Japan
• BKE13560 (ΔmtnK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACCTCCAATTATGTAA, downstream forward: _UP4_TGATCTATTCATGACCCATT
• BKK13560 (ΔmtnK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACCTCCAATTATGTAA, downstream forward: _UP4_TGATCTATTCATGACCCATT

References

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