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cshA [2019-08-15 11:06:55]
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cshA [2019-08-15 11:06:55]

DEAD-box RNA helicase, important for adaptation to low temperatures
Locus
BSU_04580
Isoelectric point
9.89
Molecular weight
57.11 kDa
Protein length
511 aa Sequence Blast
Gene length
1536 bp Sequence Blast
Function
RNA helicase
Product
DEAD-box RNA helicase
Essential
no
Synonyms
ydbR
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → RNA synthesis and degradation → DEAD-box RNA helicases
  • Information processing → Protein synthesis, modification and degradation → Translation → Ribosome assembly
  • Lifestyles → Coping with stress → Cold stress proteins
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    511,106 → 512,641

    Phenotypes of a mutant

  • poor growth at low temperatures (16 to 20°C) PubMed
  • reduced number of ribosomes PubMed
  • no expression of the frlB-frlO-frlN-frlM-frlD operon PubMed
  • strongly increased expression of the pftA-pftB operon PubMed
  • transcription profile resulting from rny depletion: GEO PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • RNA helicase
  • required for ribosome assembly (biogenesis of the large subunit) PubMed
  • unwind duplex RNA with or without overhangs PubMed
  • ATP + H2O --> ADP + H+ + phosphate (according to UniProt)

    • Protein family

  • helicase family (according to UniProt)
  • DEAD-box RNA helicases (according to UniProt)

    • Paralogous protein(s)

  • DeaD, YfmL, CshB

    • Structure

  • 5IVL PubMed

    • Localization

  • cytoplasma, colocalizes with the ribosomes PubMed, may also associate with the cell membrane PubMed

    • Expression and Regulation

    Operons

    Genes
    cshA
    Description
    PubMed
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    Biological materials

    Mutant

  • GP1035 (ΔcshA::aphA3), available in Jörg Stülke's lab PubMed
  • GP1083 (ΔcshA::cat), available in Jörg Stülke's lab
  • BKE04580 (ΔcshA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTTATAAAACTGCCCTTT, downstream forward: _UP4_TAATTTGATCGATTCAGAGC
  • BKK04580 (ΔcshA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTTATAAAACTGCCCTTT, downstream forward: _UP4_TAATTTGATCGATTCAGAGC

    • Expression vectors

  • for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, in pGP382: pGP1387, available in Jörg Stülke's lab
  • for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1026 (aphA3), available in Jörg Stülke's lab
  • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP1386, available in Jörg Stülke's lab

    • GFP fusion

  • pGP1369 for chromosomal expression of CshA-YFP, available in Jörg Stülke's lab
  • B. subtilis GP1081 cshA-gfp spc, available in Jörg Stülke's lab,
  • GP1721 (in pBP43), expression of cshA-GFP::spc under the native promoter, available in Jörg Stülke's lab PubMed

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab, PubMed

    • FLAG-tag construct

  • GP1010 (spc, based on pGP1331), available in Jörg Stülke's lab
  • GP1074 (tet), available in Jörg Stülke's lab

    • Labs working on this gene/protein

  • Mohamed Marahiel, Marburg University, Germany homepage

    • References

    Reviews

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    Original publications

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