cshA [Thu Dec 15 2016 14:10:23 GMT+0100 (CET)]

DEAD-box RNA helicase, important for adaptation to low temperatures

Locus: BSU04580

Isoelectric point: 9.00

Molecular weight: 57.00 kDa

Function: RNA helicase

Product: DEAD-box RNA helicase

Essential: no

Synonyms: ydbR

Genomic Context

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Categories containing this gene/protein

• Information processing → RNA synthesis and degradation → DEAD-box RNA helicases
• Information processing → Protein synthesis, modification and degradation → Translation → Ribosome assembly
• Lifestyles → Coping with stress → Cold stress proteins
• Groups of genes → Membrane proteins

Categories containing this gene/protein

• DEAD-box RNA helicases, translation, cold stress proteins, membrane proteins

Gene

Coordinates on the chromosome (coding sequence): 511,106 -> 512,641

Phenotypes of a mutant

• poor growth at low temperatures (16 to 20°C) PubMed
• reduced number of ribosomes PubMed
• no expression of the frlB-frlO-frlN-frlM-frlD operon PubMed
• strongly increased expression of the ysbA-ysbB operon PubMed
• transcription profile resulting from rny depletion: GEO PubMed

The protein

Catalyzed reaction/ biological activity

• RNA helicase
• required for ribosome assembly (biogenesis of the large subunit) PubMed

Protein family

• helicase C-terminal domain (according to Swiss-Prot) DEAD-box RNA helicases

Paralogous protein(s)

• CshB, DeaD, YfmL

Localization

• cytoplasma, colocalizes with the ribosomes PubMed, cell membrane PubMed

Interactions

• CshA interacts with the RNA polymerase PubMed
• part of the RNA degradosome PubMed
• CshA-CshA PubMed
• CshA-CspB PubMed
• CshA-PnpA PubMed
• CshA-Rny PubMed
• CshA-RnjA PubMed
• CshA-Eno PubMed
• CshA-PfkA PubMed
• DeaD-CshA PubMed
• RnpA-CshA PubMed
• RplA-CshA PubMed
• RplC-CshA PubMed

Expression and Regulation

Regulation

• induced by cold shock PubMed
• constitutive, similar expression at high and low temperatures, throughout growth and in both minimal and complex medium PubMed

Biological materials

Mutant

• GP1035 (aphA3), available in Jörg Stülke's lab
• GP1083 (cat), available in Jörg Stülke's lab

Expression vector

• for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, in pGP382: pGP1387, available in Jörg Stülke's lab
• for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1026 (aphA3), available in Jörg Stülke's lab
• for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP1386, available in Jörg Stülke's lab

GFP fusion

• pGP1369 for chromosomal expression of CshA-YFP, available in Jörg Stülke's lab
• B. subtilis GP1081 cshA-gfp spc, available in Jörg Stülke's lab,

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

FLAG-tag construct

• GP1010 (spc, based on pGP1331), available in Jörg Stülke's lab
• GP1074 (tet), available in Jörg Stülke's lab

Labs working on this gene/protein

• Mohamed Marahiel, Marburg University, Germany homepage

References

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