bacterial hydrophobin, forms water-repellent surface layer of the biofilm, required for sliding, inhibitor of KinA autophosphorylation, and subsequently of entry into sporulation
function
biofilm formation, control of entry into sporulation via the phosphorelay
product
biofilm surface layer, inhibitor of KinA autophosphorylation
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.2|Proteins controlling the activity of the kinases][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.3|Repellent surface layer][category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.2|Proteins controlling the activity of the kinases][category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins][SW|Categories] containing this gene/protein
[SW|biofilm formation], [SW|phosphorelay]This gene is a member of the following [SW|regulons]
[SW|AbrB regulon], [SW|DegU regulon]Gene
Coordinates on the chromosome (coding sequence)
3,187,503 -> 3,188,048
Phenotypes of a mutant
reduced colony complexity [Pubmed|21742882]loss of surface repellency of the biofilm [Pubmed|22571672]diminished [SW|sliding] of ''B. subtilis'' natto [Pubmed|26152584]The protein
Catalyzed reaction/ biological activity
inhibits [protein|search|KinA] autophophorylation [Pubmed|23335417]contributes to the surface stiffness and increases the surface roughness of NCIB 3610 bioflms [Pubmed|26873313]Paralogous protein(s)
[protein|search|SivA]Structure
[PDB|4BHU] [Pubmed|23904481][SW|Localization]
extracellular (signal peptide) [Pubmed|18957862]biofilm matrix in an extracellular polysaccharide-dependent manner [Pubmed|22571672]forms a layer on the biofilm surface [Pubmed|22571672]Expression and Regulation
Operon
''[protein|search|bslA]'' [Pubmed|22383849]Regulatory mechanism
[protein|search|AbrB]: transcription repression [Pubmed|18978066][protein|search|LutR]: transcription activation [Pubmed|24196425]expression is down-regulated in a ''[protein|search|rok]'' mutant (indirect effect) [Pubmed|21097620][protein|search|DegU]-P: transcription activation [Pubmed|18978066], this might be indirect [Pubmed|21742882]Biological materials
Mutant
''B. subtilis'' W939 ''bslA''::Cm (ATCC6051 based) [Pubmed|17850253]''B. subtilis'' NRS2097 ''bslA''::Cm (NCIB3610 based) [Pubmed|18978066]''B. subtilis'' ''bslA''::Cm (168 based) [Pubmed|21097620]Expression vector
Physpank-bslA based on vector pDR111: pDRyuaB2 [Pubmed|21097620]lacZ fusion
pNW500 P''bslA-lacZ'' fusion in pDG1663 [Pubmed|18978066]GFP fusion
P''bslA-gfp'' fusion in pSG1151 vector: pSGyuaB [Pubmed|21097620]References
Reviews
22607588,23979433,24988880,25907113,27458867 Original publications
26152584,22571672,18978066,18957862,17850253,21097620,21742882,22383849,23335417,23904481,22571672,25870300,26152584,26378478,26873313 
