bslA [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]

bacterial hydrophobin, forms water-repellent surface layer of the biofilm, required for sliding, inhibitor of KinA autophosphorylation, and subsequently of entry into sporulation

Locus: BSU31080

Isoelectric point: 9.00

Molecular weight: 19.00 kDa

Function: biofilm formation, control of entry into sporulation via the phosphorelay

Product: biofilm surface layer, inhibitor of KinA autophosphorylation

Essential: no

Synonyms: yuaB, sivB

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → phosphorelay → Proteins controlling the activity of the kinases
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Repellent surface layer
• Lifestyles → Sporulation → phosphorelay → Proteins controlling the activity of the kinases
• Groups of genes → Secreted proteins

Categories containing this gene/protein

• biofilm formation, phosphorelay

This gene is a member of the following regulons

• AbrB regulon, DegU regulon

Gene

Coordinates on the chromosome (coding sequence): 3,187,503 -> 3,188,048

Phenotypes of a mutant

• reduced colony complexity PubMed
• loss of surface repellency of the biofilm PubMed
• diminished sliding of B. subtilis natto PubMed

The protein

Catalyzed reaction/ biological activity

• inhibits KinA autophophorylation PubMed
• contributes to the surface stiffness and increases the surface roughness of NCIB 3610 bioflms PubMed

Paralogous protein(s)

• SivA

Structure

• 4BHU PubMed

Localization

• extracellular (signal peptide) PubMed
• biofilm matrix in an extracellular polysaccharide-dependent manner PubMed
• forms a layer on the biofilm surface PubMed

Expression and Regulation

Operon

• bslA PubMed

Regulatory mechanism

• AbrB: transcription repression PubMed
• LutR: transcription activation PubMed
• expression is down-regulated in a rok mutant (indirect effect) PubMed
• DegU-P: transcription activation PubMed, this might be indirect PubMed

Biological materials

Mutant

• MGNA-A218 (yuaB::erm), available at the NBRP B. subtilis, Japan
• B. subtilis W939 bslA::Cm (ATCC6051 based) PubMed
• B. subtilis NRS2097 bslA::Cm (NCIB3610 based) PubMed
• B. subtilis bslA::Cm (168 based) PubMed

Expression vector

• Physpank-bslA based on vector pDR111: pDRyuaB2 PubMed

LacZ fusion

• pNW500 PbslA-lacZ fusion in pDG1663 PubMed

GFP fusion

• PbslA-gfp fusion in pSG1151 vector: pSGyuaB PubMed

References

Reviews

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Original publications

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