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araB
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araB

L-ribulokinase
Locus
BSU_28790
Isoelectric point
5.23
Molecular weight
60.84 kDa
Protein length
560 aa Sequence Blast
Gene length
1683 bp Sequence Blast
Function
arabinose utilization
Product
L-ribulokinase
Essential
no
E.C.
2.7.1.16
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of arabinan/ arabinose/ arabitol

    • Gene

    Coordinates
    2,945,689 → 2,947,371

    The protein

    Catalyzed reaction/ biological activity

  • ATP + L(or D)-ribulose --> ADP + L(or D)-ribulose 5-phosphate + H+ (according to UniProt)

    • Protein family

  • ribulokinase family (single member, according to UniProt)

    • Structure

  • 3QDK (B. halodurans AraB)

    • Expression and Regulation

    Operons

    Genes
    araA-araB-araD-araL-araM-araN-araP-araQ-abfA
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • AraR: repression, PubMed, in AraR regulon

    • Regulation

  • induced by arabinose (AraR) PubMed

    • Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed
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    Biological materials

    Mutant

  • BKE28790 (ΔaraB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TGTGTAAGCCATGTGACATC, downstream forward: _UP4_AAGAATTGATAAAGGGTGAT
  • BKK28790 (ΔaraB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TGTGTAAGCCATGTGACATC, downstream forward: _UP4_AAGAATTGATAAAGGGTGAT

    • References

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