phoA

alkaline phosphatase A

locus: BSU_09410

pI: 9.93

mw: 50.11 kDa

protein length: 461 aa

gene length: 1386 bp

function: aquisition of phosphate upon phosphate starvation

product: alkaline phosphatase A

essential: no

ec: 3.1.3.1

synonyms: phoAIV

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.3|Phosphate metabolism]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 1,017,083 → 1,018,468

The protein

Catalyzed reaction/ biological activity

• phosphate monoester + H₂O --> alcohol + phosphate (according to UniProt)

Protein family

• alkaline phosphatase family (with [protein|3B95AB3021280215B6B487A348D78FB5797AEEA6|PhoB], according to UniProt)

Paralogous protein(s)

• [protein|3B95AB3021280215B6B487A348D78FB5797AEEA6|PhoB]

Structure

• [PDB|3A52] (from Shewanella sp., mature protein without signal peptide, 40% identity) [pubmed|20057143]

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|E0430C16C41440633647976A0F9183E24268E198|phoA]
  description: [Pubmed|16291680]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8113174], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|15699190], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulatory mechanism

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [Pubmed|16291680,8113174], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]

  regulation

  • expressed under conditions of phosphate limitation ([protein|search|PhoP]) [Pubmed|16291680,8113174]

Biological materials

Mutant

• 1A767 ( ''phoA''::''cat''), [Pubmed|9426145], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A767&Search=1A767 BGSC]
• BKE09410 (Δ[gene|E0430C16C41440633647976A0F9183E24268E198|phoA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCCTCCTTTAATGTATT, downstream forward: _UP4_TAAAAAGGATGCAGAACGCT
• BKK09410 (Δ[gene|E0430C16C41440633647976A0F9183E24268E198|phoA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCCTCCTTTAATGTATT, downstream forward: _UP4_TAAAAAGGATGCAGAACGCT

labs

• [SW|Marion Hulett], University of Illinois at Chicago, USA [http://www.uic.edu/depts/bios/faculty/hulett/hulettm.shtml Homepage]

References

• 9593301,10913081,16491025,15699190,18957862,16291680,8113174,25666134,27118079,20057143