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phoA
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phoA

alkaline phosphatase A
Locus
BSU_09410
Isoelectric point
9.93
Molecular weight
50.11 kDa
Protein length
461 aa Sequence Blast
Gene length
1386 bp Sequence Blast
Function
aquisition of phosphate upon phosphate starvation
Product
alkaline phosphatase A
Essential
no
E.C.
3.1.3.1
Synonyms
phoAIV

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,017,083 → 1,018,468

The protein

Catalyzed reaction/ biological activity

  • phosphate monoester + H2O --> alcohol + phosphate (according to UniProt)
  • Protein family

  • alkaline phosphatase family (with PhoB, according to UniProt)
  • Paralogous protein(s)

    Structure

  • 3A52 (from Shewanella sp., mature protein without signal peptide, 40% identity) PubMed
  • Localization

  • extracellular (signal peptide) PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigG: sigma factor, PubMed, in SigG regulon
  • Regulatory mechanism

  • PhoP: activation, PubMed, in PhoP regulon
  • Regulation

  • expressed under conditions of phosphate limitation (PhoP) PubMed
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    Biological materials

    Mutant

  • 1A767 ( phoA::cat), PubMed, available at BGSC
  • BKE09410 (ΔphoA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCCTCCTTTAATGTATT, downstream forward: _UP4_TAAAAAGGATGCAGAACGCT
  • BKK09410 (ΔphoA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCCTCCTTTAATGTATT, downstream forward: _UP4_TAAAAAGGATGCAGAACGCT
  • Labs working on this gene/protein

  • Marion Hulett, University of Illinois at Chicago, USA Homepage
  • References

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