phoA

alkaline phosphatase A

Locus: BSU_09410

Isoelectric point: 9.93

Molecular weight: 50.11 kDa

Protein length: 461 aa

Gene length: 1386 bp

Function: aquisition of phosphate upon phosphate starvation

Product: alkaline phosphatase A

Essential: no

E.C.: 3.1.3.1

Synonyms: phoAIV

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Phosphate metabolism
• Groups of genes → Secreted proteins

Gene

Coordinates: 1,017,083 → 1,018,468

The protein

Catalyzed reaction/ biological activity

• phosphate monoester + H₂O --> alcohol + phosphate (according to UniProt)

Protein family

• alkaline phosphatase family (with PhoB, according to UniProt)

Paralogous protein(s)

• PhoB

Structure

• 3A52 (from Shewanella sp., mature protein without signal peptide, 40% identity) PubMed

Localization

• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: phoA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigG: sigma factor, PubMed, in SigG regulon

  Regulatory mechanism

  • PhoP: activation, PubMed, in PhoP regulon

  Regulation

  • expressed under conditions of phosphate limitation (PhoP) PubMed

Biological materials

Mutant

• 1A767 ( phoA::cat), PubMed, available at BGSC
• BKE09410 (ΔphoA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCCTCCTTTAATGTATT, downstream forward: _UP4_TAAAAAGGATGCAGAACGCT
• BKK09410 (ΔphoA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCCTCCTTTAATGTATT, downstream forward: _UP4_TAAAAAGGATGCAGAACGCT

Labs working on this gene/protein

• Marion Hulett, University of Illinois at Chicago, USA Homepage

References

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