SubtiBank SubtiBank
prkD [2017-8-11 16:0:3]
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prkD [2017-8-11 16:0:3]

protein kinase D
Locus
BSU02030
Isoelectric point
6.36
Molecular weight
29.91 kDa
Protein length
256 aa Sequence Blast
Gene length
768 bp Sequence Blast
Function
unknown
Product
protein kinase D
Essential
no
E.C.
2.7.11.1
Synonyms
ybdM
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Protein modification → Protein kinases
  • Groups of genes → Phosphoproteins → Phosphorylation on a Thr residue

    • Gene

    Coordinates
    223,219 → 223,989

    The protein

    Catalyzed reaction/ biological activity

  • ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
  • phosphorylates DegS on Ser-76 (in vitro) PubMed

    • Protein family

  • protein kinase domain (according to Swiss-Prot)

    • Modification

  • autophosphorylated on Thr-174 (in vitro) PubMed

    • Biological materials

    Mutant

  • MGNA-B958 (ybdM::erm), available at the NBRP B. subtilis, Japan
  • GP578 (aphA3), available in the Stülke lab

    • Expression vector

  • pGP390 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP821, available in Stülke lab, PubMed
  • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP1407, available in Stülke lab

    • LacZ fusion

  • pGP830 (in pAC7), available in Stülke lab

    • References

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