prkD [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]

protein kinase D

locus: BSU02030

pI: 6.36

mw: 29.00 kDa

function: unknown

product: protein kinase D

essential: no

ec: 2.7.11.1

synonyms: ybdM

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]

[SW|Categories] containing this gene/protein

• [SW|protein modification], [SW|phosphoproteins]

Gene

Coordinates on the chromosome (coding sequence): 223,219 -> 223,989

The protein

Catalyzed reaction/ biological activity

• ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
• phosphorylates [protein|search|DegS] on Ser-76 (in vitro) [Pubmed|21304896]

Protein family

• [SW|protein kinase] domain (according to Swiss-Prot)

Modification

• autophosphorylated on Thr-174 (''in vitro'') [Pubmed|20389117]

[SW|Interactions]

• [protein|search|AbrB]-[protein|search|PrkD] [Pubmed|24731262]

Biological materials

Mutant

• GP578 (aphA3), available in the [SW|Stülke] lab

Expression vector

• pGP390 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Stülke] lab
• for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP821, available in [SW|Stülke] lab
• for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP1407, available in [SW|Stülke] lab

lacZ fusion

• pGP830 (in [protein|search|pAC7]), available in [SW|Stülke] lab

References

• 20389117,12406230,21304896,24731262,25278935