ydhD

spore coat peptidoglycan hydrolase

locus: BSU_05710

pI: 9.07

mw: 48.80 kDa

protein length: 420 aa

gene length: 1263 bp

function: unknown

product: spore coat glycosylase

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.6|Cell wall/ other/ based on similarity]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.8|Not yet assigned]

Gene

Coordinates: 616,672 → 617,934

The protein

Protein family

• [SW|Glycosyl hydrolase 18 family] (according to UniProt)
• Chitinase class II subfamily (with [protein|77BEC74867422AA225A1F12AD63464656DBDCBE8|YaaH], according to UniProt)

Paralogous protein(s)

• [protein|77BEC74867422AA225A1F12AD63464656DBDCBE8|YaaH]

[SW|Domains]

• contains two N-acetylglucosamine-polymer-binding [SW|LysM domain]s at the N-terminus [Pubmed|18430080]
• [SW|glycoside hydrolase family 18 domain ] (aa 120 to 400)
• 2 [SW|LysM domain]s (aa 2-45, aa 48-92) (according to UniProt)

Structure

• [PDB|3CZ8]

[SW|Localization]

• spore wall (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|DFA0BFED9FC2C47ABAA2284514CE2F81BD9EDFEF|ydhD]
  description: [Pubmed|11011148]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|11011148,15699190], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulation

  • expressed during sporulation ([protein|search|SigE])

Biological materials

Mutant

• MGNA-C179 (ydhD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2177 NBRP B. subtilis, Japan]
• BKE05710 (Δ[gene|DFA0BFED9FC2C47ABAA2284514CE2F81BD9EDFEF|ydhD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTAACCCCCATTATT, downstream forward: _UP4_TAAAAAAAGACACCAGAGCT
• BKK05710 (Δ[gene|DFA0BFED9FC2C47ABAA2284514CE2F81BD9EDFEF|ydhD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTAACCCCCATTATT, downstream forward: _UP4_TAAAAAAAGACACCAGAGCT

References

Reviews

• 18430080

Original publications

• 11011148,15699190