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sftA [2019-09-04 09:15:26]
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sftA [2019-09-04 09:15:26]

DNA translocase, translocation of non-segregated chromosomes prior to septum closure
locus
BSU_29805
pI
5.00
mw
107.00 kDa
protein length
952 aa Sequence Blast
gene length
2859 bp Sequence Blast
function
resolution of chromosome dimers
product
DNA translocase
essential
no
synonyms
ytpS,cdtA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]

    • Gene

    Coordinates
    3,049,725 → 3,052,583

    The protein

    Catalyzed reaction/ biological activity

  • aids in moving DNA away from the closing septum, binds DNA, DNA-dependent ATPase activity [Pubmed|19818024]
  • translocation of non-segregated chromosomes prior to septum closure
  • the two DNA translocases [protein|DF77B46666595E86081C5345C91E08F86DF9FAEE|SftA] and [protein|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|SpoIIIE] synergistically affect chromosome dimer resolution presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively [Pubmed|21239579]

    • Protein family

  • FtsK/SpoIIIE/SftA family (with [protein|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|SpoIIIE], according to UniProt)

    • Paralogous protein(s)

  • [protein|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|SpoIIIE]

    • Structure

  • [PDB|2IUT] (FtsK from ''Pseudomonas aeruginosa'', partial, 48% identity, complex with [protein|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|SpoIIIE]) [Pubmed|16916635]

    • [SW|Localization]

  • 30% of the molecules in the cytoplasm (Homogeneous) [Pubmed|29439991,28110333,16479537]
  • small fraction at the membrane [Pubmed|28110333]
  • 70% of the molecules are septum-bound, co-localizes with [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|FtsZ] at nascent division sites, recruitment to the septum depends on [protein|search|FtsA ][Pubmed|29439991,19788545]

    • Expression and Regulation

    Operons

    genes
    [gene|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA]-[gene|3BF530AB1B6A3DB3671FC046E2B38CA1936E6F78|murC]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-A438 (ytpS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/438 NBRP B. subtilis, Japan]
  • 1A984 ( ''sftA''::''spec''), [Pubmed|19788545], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A984&Search=1A984 BGSC]
  • BKE29805 (''[gene|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA]''::''erm'', available in the BGSC, in [SW|Fabian Commichau]'s, and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
  • BKE29805 (Δ[gene|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29805 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATTTATCTCTAACTCC, downstream forward: _UP4_TAATATGGGTTCATTTTCAC
  • BKK29805 (Δ[gene|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29805 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATTTATCTCTAACTCC, downstream forward: _UP4_TAATATGGGTTCATTTTCAC

    • References

    Reviews

  • 22047950,22934648

    • Original publications

  • 29439991,16479537,19788545,19818024,21239579,16916635,27891124,28110333