yceD [2018-05-28 18:10:42]

general stress protein, similar to tellurium resistance protein

locus: BSU02900

pI: 4.29

mw: 20.55 kDa

protein length: 193 aa

gene length: 579 bp

function: required for survival of ethanol stress

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.12|Resistance against toxic metals/ based on similarity]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 312,780 → 313,361

The protein

Protein family

• CAPAB/terDEXZ family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|7E4742F30B4C43FBF9DAE0B02948022AE7912A33|YceC], [protein|863E26D497D29B820912A0C0F4A3AE4C6865EC2E|YceE]

Structure

• [PDB|2KXT] (from Klebsiella pneumoniae, 59% identity)

[SW|Localization]

• membrane associated [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|7E4742F30B4C43FBF9DAE0B02948022AE7912A33|yceC]-[gene|DF30A8B195B8D830E131B23C768F07BE61F51150|yceD]-[gene|863E26D497D29B820912A0C0F4A3AE4C6865EC2E|yceE]-[gene|F16A11027D8D320D3D64E44038E29921A946955B|yceF]-[gene|F588108F12C5CA855A24159F28223EF67145D28F|yceG]-[gene|3A9EB32D5627FAB3F7646B7918E54C8D1ADD25DF|yceH]
  description: [Pubmed|11866510]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|11866510], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|11544224,15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX]: sigma factor, [Pubmed|19047346], in [regulon|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX regulon]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|11544224]

• genes: [gene|7E4742F30B4C43FBF9DAE0B02948022AE7912A33|yceC]-[gene|DF30A8B195B8D830E131B23C768F07BE61F51150|yceD]-[gene|863E26D497D29B820912A0C0F4A3AE4C6865EC2E|yceE]-[gene|F16A11027D8D320D3D64E44038E29921A946955B|yceF]
  description: [Pubmed|11866510]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [pubmed|11866510], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [pubmed|11544224,15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX]: sigma factor, [pubmed|19047346], in [regulon|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX regulon]

  regulation

  • induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]) [Pubmed|11544224]

Biological materials

Mutant

• MGNA-B984 (yceD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1983 NBRP B. subtilis, Japan]
• BKE02900 (Δ[gene|DF30A8B195B8D830E131B23C768F07BE61F51150|yceD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCATCCACTCCTTT, downstream forward: _UP4_TAAGACAAATACGAAGAGCA
• BKK02900 (Δ[gene|DF30A8B195B8D830E131B23C768F07BE61F51150|yceD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCATCCACTCCTTT, downstream forward: _UP4_TAAGACAAATACGAAGAGCA

References

• 15805528,18763711,11866510,18179421,19047346