fadF

similar to iron-sulphur-binding reductase

locus: BSU_37180

pI: 6.53

mw: 79.00 kDa

protein length: 705 aa

gene length: 2118 bp

function: fatty acid degradation

product: unknown

essential: no

synonyms: ywjF

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.3|Utilization of fatty acids]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,814,382 → 3,816,499

The protein

[SW|Domains]

• 2 [SW|4Fe-4S ferredoxin-type domain]s (aa 268-298, aa 360-391) (according to UniProt)

[SW|Cofactors]

• 2 4Fe-4S clusters [pubmed|29292548]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|DEE0DA15EE9EA27BA8C332204E40D336324A111C|fadF]-[gene|7D89BBB52403BF99416250688EFA90C2BE8EB591|acdA]-[gene|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE]
  description: [Pubmed|17189250]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|17189250], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]: repression, [Pubmed|17189250], in [regulon|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR regulon]

  regulation

  • repressed in the absence of long-chain fatty acids ([protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]) [Pubmed|17189250]

Biological materials

Mutant

• MGNA-A555 (ywjF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/555 NBRP B. subtilis, Japan]
• BKE37180 (''[gene|DEE0DA15EE9EA27BA8C332204E40D336324A111C|fadF]''::''erm'', available in the BGSC, in [SW|Fabian Commichau]'s, and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
• BKE37180 (Δ[gene|DEE0DA15EE9EA27BA8C332204E40D336324A111C|fadF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCAATTCCCCCTTTTT, downstream forward: _UP4_GATCTGAAAATGGGGGAGAA
• BKK37180 (Δ[gene|DEE0DA15EE9EA27BA8C332204E40D336324A111C|fadF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCAATTCCCCCTTTTT, downstream forward: _UP4_GATCTGAAAATGGGGGAGAA

References

• 12850135,17189250,23033921