SubtiBank SubtiBank
fadF
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fadF

similar to iron-sulphur-binding reductase
Locus
BSU_37180
Isoelectric point
6.53
Molecular weight
79.00 kDa
Protein length
705 aa Sequence Blast
Gene length
2118 bp Sequence Blast
Function
fatty acid degradation
Product
unknown
Essential
no
Synonyms
ywjF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Lipid metabolism → Utilization of lipids → Utilization of fatty acids
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    3,814,382 → 3,816,499

    The protein

    Domains

  • 2 4Fe-4S ferredoxin-type domains (aa 268-298, aa 360-391) (according to UniProt)

    • Cofactors

  • 2 4Fe-4S clusters PubMed

    • Localization

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    Genes
    fadF-acdA-rpoE
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • FadR: repression, PubMed, in FadR regulon

    • Regulation

  • repressed in the absence of long-chain fatty acids (FadR) PubMed
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    Biological materials

    Mutant

  • MGNA-A555 (ywjF::erm), available at the NBRP B. subtilis, Japan
  • BKE37180 (fadF::erm, available in the BGSC, in Fabian Commichau's, and in Jörg Stülke's lab) PubMed
  • BKE37180 (ΔfadF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCAATTCCCCCTTTTT, downstream forward: _UP4_GATCTGAAAATGGGGGAGAA
  • BKK37180 (ΔfadF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCAATTCCCCCTTTTT, downstream forward: _UP4_GATCTGAAAATGGGGGAGAA

    • References

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