bstG

bacillithiol S-transferase, nuclease inhibitor

locus: BSU_05630

pI: 5.61

mw: 19.76 kDa

protein length: 172 aa

gene length: 519 bp

function: response to DNA damage

product: bacillithiol S-transferase, nuclease inhibitor

essential: no

synonyms: dinB

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

Gene

Coordinates: 608,246 → 608,764

The protein

Protein family

• [SW|S-transferase-like (STL) superfamily] [pubmed|29451913]
• [SW|DinB family] (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|DEB7605B4F6F5F365A49C1C15647F9767B01BF66|bstG]
  description: [Pubmed|1847907]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [PubMed|8226626,8969214], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]

  regulation

  • induced by DNA damage ([protein|search|LexA]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8226626,8969214 PubMed]

  additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

Biological materials

Mutant

• BKE05630 (Δ[gene|DEB7605B4F6F5F365A49C1C15647F9767B01BF66|bstG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTAAAATTCCCCCTTT, downstream forward: _UP4_TAAAAAAGCGGCTCCCTAAA
• BKK05630 (Δ[gene|DEB7605B4F6F5F365A49C1C15647F9767B01BF66|bstG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTAAAATTCCCCCTTT, downstream forward: _UP4_TAAAAAAGCGGCTCCCTAAA

References

• 12884008,1847907,8226626,8969214,9555905,16267290,29451913