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amiE
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amiE

N-acetylmuramyl-L-alanine amidase
Locus
BSU_01670
Isoelectric point
9.35
Molecular weight
49.14 kDa
Protein length
441 aa Sequence Blast
Gene length
1326 bp Sequence Blast
Function
recycling of cell wall material
Product
N-acetylmuramyl-L-alanine amidase
Essential
no
Synonyms
ybbE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Utilization of cell wall components

    • Gene

    Coordinates
    188,408 → 189,733

    The protein

    Catalyzed reaction/ biological activity

  • hydrolyzes the amide bond between MurNAc and the L-alanine residue of the stem peptide PubMed
  • Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides

    • Protein family

  • peptidase S12 family (single member, according to UniProt)

    • Structure

  • 4IVK (from uncultured bacterium, 25% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    murQ-murR-murP-amiE-nagZ-ybbC
    Description
    PubMed

    Regulatory mechanism

  • MurR: repression, PubMed, in MurR regulon

    • Regulation

  • expressed in late exponential and early stationary phase PubMed
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    Biological materials

    Mutant

  • BKE01670 (ΔamiE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAGGCTAGCCCCTTTCTG, downstream forward: _UP4_TGAATGATTAGACGATAGGA
  • BKK01670 (ΔamiE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAGGCTAGCCCCTTTCTG, downstream forward: _UP4_TGAATGATTAGACGATAGGA

    • References

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