amyE [2019-07-22 10:32:59]

alpha-amylase

locus: BSU_03040

pI: 5.85

mw: 72.62 kDa

protein length: 660 aa

gene length: 1980 bp

function: starch degradation

product: alpha-amylase

essential: no

ec: 3.2.1.1

synonyms: amyA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.23|Utilization of starch/ maltodextrin]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 327,618 → 329,597

The protein

Catalyzed reaction/ biological activity

• Endohydrolysis of (1→4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides (according to Swiss-Prot)

Protein family

• [SW|glycosyl hydrolase 13 family] (according to UniProt)

Structure

• [PDB|1BAG] (complex with maltopentaose)

[SW|Localization]

• secreted (according to Swiss-Prot), extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|E48DE0C73CB859E2B367B6800E85BD94836390A5|ycgB]-[gene|DE4857E8A228F145EFB9B7AD817585258344ED1F|amyE]
  description: [Pubmed|22383849]

• genes: [gene|DE4857E8A228F145EFB9B7AD817585258344ED1F|amyE]
  description: [Pubmed|11320136]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|3123701], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|7665492], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: activation, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • repressed by glucose ([protein|search|CcpA]) [Pubmed|7665492]

Biological materials

Mutant

• GP550 (cat), available in [SW|Stülke] lab
• BKE03040 (Δ[gene|DE4857E8A228F145EFB9B7AD817585258344ED1F|amyE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTGACACTCCTTATT, downstream forward: _UP4_TGAGGGCAAGGCTAGACGGG
• BKK03040 (Δ[gene|DE4857E8A228F145EFB9B7AD817585258344ED1F|amyE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTGACACTCCTTATT, downstream forward: _UP4_TGAGGGCAAGGCTAGACGGG

References

Reviews

• 28724440

Original publications

• 1904524,3123701,18957862,21948839,23262127,22900538,20817675,21512239,25431404