amyE [Mon Aug 31 2015 13:52:14 GMT+0200 (CEST)]

alpha-amylase

locus: BSU03040

pI: 5.85

mw: 72.00 kDa

function: starch degradation

product: alpha-amylase)

essential: no

ec: 3.2.1.1

synonyms: amyA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.23|Utilization of starch/ maltodextrin]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

syns: amyA

[SW|Categories] containing this gene/protein

• [SW|utilization of specific carbon sources]

This gene is a member of the following [SW|regulons]

• [SW|AbrB regulon], [SW|CcpA regulon]

The protein

Catalyzed reaction/ biological activity

• Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides (according to Swiss-Prot)

Protein family

• glycosyl hydrolase 13 family (according to Swiss-Prot)

Structure

• [PDB|1BAG] (complex with maltopentaose)

[SW|Localization]

• secreted (according to Swiss-Prot), extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operon

• ''[protein|search|amyE]'' [Pubmed|11320136]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|3123701]

Regulation

• repressed by glucose ([protein|search|CcpA]) [Pubmed|7665492]

Regulatory mechanism

• [protein|search|CcpA]: transcription repression [Pubmed|7665492]
• [protein|search|AbrB]: transcription activation [Pubmed|20817675]

Biological materials

Mutant

• GP550 (cat), available in [SW|Stülke] lab

References

• 1904524,3123701,18957862,21948839,23262127,22900538,20817675,21512239,25431404