ylbK

similar to patatin-like phospholipase

locus: BSU_15040

pI: 9.16

mw: 28.11 kDa

protein length: 260 aa

gene length: 783 bp

function: unknown

product: putative phospholipase

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 1,571,981 → 1,572,763

The protein

Protein family

• NTE family (single member, according to UniProt)

[SW|Domains]

• PNPLA domain (aa 8-166) (according to UniProt)

Structure

• [PDB|5FYA] (from Pseudomonas aeruginosa, 35% identity) [pubmed|27012424]

Expression and Regulation

Operons

• genes: [gene|DE3BCF20DE5BB438ACBB36B412817F6124CFDFFC|ylbK]-[gene|1A5C1BDE844AA56B90C0E4A02D74978676487E99|ddcP]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-B247 (ylbK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1246 NBRP B. subtilis, Japan]
• BKE15040 (Δ[gene|DE3BCF20DE5BB438ACBB36B412817F6124CFDFFC|ylbK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAGCGTTAACCTCCTGCC, downstream forward: _UP4_ATAGAGAATTGGGAGGGCTC
• BKK15040 (Δ[gene|DE3BCF20DE5BB438ACBB36B412817F6124CFDFFC|ylbK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAGCGTTAACCTCCTGCC, downstream forward: _UP4_ATAGAGAATTGGGAGGGCTC

References

• 20525796,27012424