thiL [2019-07-23 11:13:25]

thiamine-monophosphate kinase

locus: BSU_05900

pI: 5.04

mw: 35.73 kDa

protein length: 325 aa

gene length: 978 bp

function: biosynthesis of thiamine

product: thiamine-monophosphate kinase

essential: no

ec: 2.7.4.16

synonyms: ydiA,ydxA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.3|Biosynthesis/ acquisition of thiamine]

Gene

Coordinates: 640,662 → 641,639

The protein

Catalyzed reaction/ biological activity

• ATP + thiamine phosphate = ADP + thiamine diphosphate (according to Swiss-Prot)

Protein family

• thiamine-monophosphate kinase family (single member, according to UniProt)

Structure

• [PDB|1VQV] (from ''Aquifex aeolicus'', 33% similarity, 51% identity)

Expression and Regulation

Operons

• genes: [gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]-[gene|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]-[gene|6CB350C335603D5F4434679F9D740BFECA738599|tsaB]-[gene|C2283BDD806221A285A27F24F52F92F4D47F0C55|ydiD]-[gene|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-C197 (ydiA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2195 NBRP B. subtilis, Japan]
• BKE05900 (Δ[gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACCAAAACCTCCTTT, downstream forward: _UP4_TTTAAATAAGACGGGTGACT
• BKK05900 (Δ[gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACCAAAACCTCCTTT, downstream forward: _UP4_TTTAAATAAGACGGGTGACT

References

Reviews

• 19348578

Original publications

• 16291685,22383849