glcF

probable glycolate oxidase, iron-sulfur subunit

locus: BSU_28690

pI: 6.45

mw: 49.04 kDa

protein length: 444 aa

gene length: 1335 bp

function: unknown

product: probable glycolate oxidase, iron-sulfur subunit

essential: no

synonyms: ysfD, glcF

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 2,934,594 → 2,935,928

The protein

[SW|Domains]

• 2 [SW|4Fe-4S ferredoxin-type domain]s (aa 14-46, aa 69-100) (according to UniProt)

[SW|Cofactors]

• two Fe-S clusters [pubmed|29292548]

Expression and Regulation

Operons

• genes: [gene|386BB8A42AF9FEB27FA3CABEDA21AA153A544316|glcD]-[gene|DDD01D9C50BC11A50D263946ECA15CB58570ACAA|glcF]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B005 (ysfD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1004 NBRP B. subtilis, Japan]
• BKE28690 (Δ[gene|DDD01D9C50BC11A50D263946ECA15CB58570ACAA|glcF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTCATCTTTCCGCCACCAC, downstream forward: _UP4_TGAGAAAGCCCAAAACAGAC
• BKK28690 (Δ[gene|DDD01D9C50BC11A50D263946ECA15CB58570ACAA|glcF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTCATCTTTCCGCCACCAC, downstream forward: _UP4_TGAGAAAGCCCAAAACAGAC

References

• 8969504,27264531,8606183