oppC

oligopeptide ABC transporter (permease)

Locus: BSU_11450

Isoelectric point: 10.11

Molecular weight: 33.47 kDa

Protein length: 305 aa

Gene length: 918 bp

Function: initiation of sporulation, competence development

Product: oligopeptide ABC transporter (permease)

Essential: no

Synonyms: spo0KC

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Importers → Uptake of peptides
• Metabolism → Amino acid/ nitrogen metabolism → Utilization of nitrogen sources other than amino acids → Utilization of peptides
• Information processing → Genetics → Genetic competence
• Information processing → Regulation of gene expression → phosphorelay
• Lifestyles → Exponential and early post-exponential lifestyles → Genetic competence
• Lifestyles → Sporulation → phosphorelay
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,222,533 → 1,223,450

The protein

Catalyzed reaction/ biological activity

• required for the uptake of quorum sensing peptides such as PhrH PubMed

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)
• OppBC subfamily (according to UniProt)

Paralogous protein(s)

• DppC, AppC

Domains

• ABC transmembrane type-1 domain (aa 103-292) (according to UniProt)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: oppA-oppB-oppC-oppD-oppF
  Description: PubMed

  Regulatory mechanism

  • TnrA: activation, PubMed, in TnrA regulon
  • CodY: repression, no derepression occurs, however, in a CodY mutant, due to increased repression by ScoC PubMed, in CodY regulon
  • ScoC: repression, PubMed, in ScoC regulon

  Regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

  Additional information

  • A ncRNA is predicted between 'trpS' and 'oppA' PubMed

Biological materials

Mutant

• BP68 (spc) available in Fabian Commichau's lab
• GP2097 (D(oppA-oppB-oppC-oppD-oppF)::aphA3), available in Jörg Stülke's lab
• BKE11450 (ΔoppC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGCCTAGGCTCCTTTCT, downstream forward: _UP4_GATCCTAAGTTACGTAAATA
• BKK11450 (ΔoppC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGCCTAGGCTCCTTTCT, downstream forward: _UP4_GATCCTAAGTTACGTAAATA

References

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