SubtiBank SubtiBank
yhcZ [2018-02-10T14:09:53.000Z]
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yhcZ [2018-02-10T14:09:53.000Z]

two-component response regulator
locus
BSU09330
pI
5.90
mw
23.88 kDa
protein length
214 aa Sequence Blast
gene length
642 bp Sequence Blast
function
unknown
product
two-component response regulator
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]

    • Gene

    Coordinates
    1,009,804 → 1,010,448

    The protein

    Paralogous protein(s)

  • [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|LiaR], [protein|EFEAF09E4449A022A7323450FDED9458426A0080|YdfI], [protein|387EF370CE24F7A3C20789A57329A02EBED46F53|LnrK], [protein|23F365C23BDEE02D42C9355FC7D2A65DAF9F79ED|YxjL]

    • Modification

  • phosphorylated on a Asp residue by [protein|8D570AEE5D78A9A3CDBB1F457630BAF5DB59BCFD|YhcY]

    • Structure

  • [PDB|5HEV] ([protein|search|LiaR ]from Enterococcus faecium, 44% identity) [pubmed|27670715]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|8D570AEE5D78A9A3CDBB1F457630BAF5DB59BCFD|yhcY]-[gene|DD799ED3EB79457C27ED30C7B4DBBC2C8F962191|yhcZ]-[gene|345EC248C0ADF48B0C25B175241AAE982E0E9B78|yhdA]
    description
    [Pubmed|16816187]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16816187], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|LiaR]: activation, [Pubmed|16816187], in [regulon|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|LiaR regulon]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A730 (yhcZ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/730 NBRP B. subtilis, Japan]
  • BKE09330 (Δ[gene|DD799ED3EB79457C27ED30C7B4DBBC2C8F962191|yhcZ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATACCGCCCCTCCTTT, downstream forward: _UP4_AAATGAACATGTTAGTCATA
  • BKK09330 (Δ[gene|DD799ED3EB79457C27ED30C7B4DBBC2C8F962191|yhcZ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATACCGCCCCTCCTTT, downstream forward: _UP4_AAATGAACATGTTAGTCATA

    • References

  • 10094672,20639339,16816187,27670715