comGF

DNA transport machinery

Locus: BSU_24680

Isoelectric point: 5.55

Molecular weight: 14.14 kDa

Protein length: 127 aa

Gene length: 384 bp

Function: genetic competence, DNA uptake

Product: DNA transport machinery

Essential: no

Synonyms

Genomic Context

Loading

Categories containing this gene/protein

• Information processing → Genetics → Genetic competence
• Lifestyles → Exponential and early post-exponential lifestyles → Genetic competence
• Groups of genes → Membrane proteins

Gene

Coordinates: 2,556,512 → 2,556,895

The protein

Localization

• integral membrane protein PubMed

Expression and Regulation

Operons

• Genes: comGA-comGB-comGC-comGD-comGE-comGF-comGG-spoIIIL
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigF: sigma factor, PubMed, in SigF regulon

  Regulatory mechanism

  • ComK: activation, PubMed, in ComK regulon

  Regulation

  • expressed early during sporulation in the forespore (SigF) PubMed

  Additional information

  • the comGA-comGB-comGC-comGD-comGE-comGF-comGG-spoIIIL operon requires NusA for expression Reference

Biological materials

Mutant

• BKE24680 (ΔcomGF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAAAAGACTGAGAGCGAAA, downstream forward: _UP4_TGAATGTATCGTACAAGAGG
• BKK24680 (ΔcomGF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAAAAGACTGAGAGCGAAA, downstream forward: _UP4_TGAATGTATCGTACAAGAGG
• BP914 (Δ(comGA-comGB-comGC-comGD-comGE-comGF-comGG)::phleo), available in Fabian Commichau's lab

References

Loading