metS

methionyl-tRNA synthetase

locus: BSU_00380

pI: 4.96

mw: 76.01 kDa

protein length: 664 aa

gene length: 1995 bp

function: translation

product: methionyl-tRNA synthetase

essential: yes

ec: 6.1.1.10

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

Gene

Coordinates: 45,633 → 47,627

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP + L-methionine + tRNA^Met --> AMP + diphosphate + L-methionyl-tRNA^Met (according to UniProt)

Protein family

• [SW|Class-I aminoacyl-tRNA synthetase family] (according to UniProt)

[SW|Domains]

• [SW|tRNA-binding domain] (aa 570-662) (accordinig to UniProt)

Modification

• phosphorylated on Ser-652 [Pubmed|20509597]

Structure

• [PDB|1WE0] (from ''Thermus thermophilus hb8 mutant'', 40% identity, 57% similarity) [Pubmed|10673435]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|DCBDD135F295B04FE7C7EB92E94638701CB320DD|metS]-[gene|00798BB5A769C464CC5FDDF7D9E41D30B473B9A8|yabD]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

  regulation

  • repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

  additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of [gene|DCBDD135F295B04FE7C7EB92E94638701CB320DD|metS] [Pubmed|27120414]

• genes: [gene|DCBDD135F295B04FE7C7EB92E94638701CB320DD|metS]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

  regulation

  • repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

  additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of [gene|DCBDD135F295B04FE7C7EB92E94638701CB320DD|metS] [Pubmed|27120414]

References

• 14651647,20509597,27120414,11732605