ung

uracil-DNA glycosylase

Locus: BSU_37970

Isoelectric point: 8.78

Molecular weight: 25.90 kDa

Protein length: 225 aa

Gene length: 678 bp

Function: DNA repair

Product: uracil-DNA glycosylase

Essential: no

Synonyms: ipa-57d,ywdG

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA repair/ recombination
• Information processing → Genetics → DNA repair/ recombination → Other proteins

Gene

Coordinates: 3,897,685 → 3,898,362

Phenotypes of a mutant

• increased mutation rates PubMed

The protein

Catalyzed reaction/ biological activity

• removes uracil preferentially from single-stranded DNA over double-stranded DNA, exhibiting higher preference for U:G than U:A mismatches PubMed

Protein family

• uracil-DNA glycosylase (UDG) superfamily (single member, according to UniProt)

Structure

• 3A7N (from Mycobacterium tuberculosis, 42% identity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ung
  Description: PubMed

  Regulation

  • expressed throughout growth and staionary phase PubMed

• Genes: ywdF-ung
  Description: PubMed

Biological materials

Mutant

• BKE37970 (Δung::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAGCTGTTTCAAGATTCAGG, downstream forward: _UP4_TAAAAAGCGCAGGTGATCTG
• BKK37970 (Δung::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAGCTGTTTCAAGATTCAGG, downstream forward: _UP4_TAAAAAGCGCAGGTGATCTG

References

Reviews

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Original publications

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