wprA [2018-09-13 18:15:53]

secreted quality control protease

Locus: BSU10770

Isoelectric point: 9.58

Molecular weight: 96.29 kDa

Protein length: 894 aa

Gene length: 2682 bp

Function: protein quality control

Product: secreted quality control protease

Essential: no

Synonyms: yisM

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall/ other
• Information processing → Protein synthesis, modification and degradation → Proteolysis → Protein quality control
• Groups of genes → Secreted proteins

Gene

Coordinates: 1,153,789 → 1,156,473

The protein

Catalyzed reaction/ biological activity

• important for TatAY-TatCY-dependent export of YkuE and EfeB PubMed
• involved in degradation of PrsA, HtrA, and HtrB PubMed

Protein family

• peptidase S8 family (according to UniProt)

Domains

• peptidase S8 domain (aa 458 ... 729) (according to UniProt)

Structure

• 1DBI (the peptidase S8 domain, aa 458 ... 687, 46% identity)

Localization

• extracellular (signal peptide) PubMed
• membrane PubMed
• cell wall PubMed

Expression and Regulation

Operons

• Genes: wprA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • YvrHb: activation, PubMed, in YvrHb regulon

  Regulation

  • repressed by glucose (CcpA) PubMed

  Additional information

  • An [ncRNA|search|antisense RNA] is predicted for'wprA' PubMed

Biological materials

Mutant

• 1A1021 ( wprA::kan), PubMed, available at BGSC
• BKE10770 (ΔwprA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTATCCCTCCTGCAAA, downstream forward: _UP4_TAACCAAAAAGCGGTGCTCG
• BKK10770 (ΔwprA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTATCCCTCCTGCAAA, downstream forward: _UP4_TAACCAAAAAGCGGTGCTCG

References

Reviews

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Original publications

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