ganA

beta-galactosidase

locus: BSU_34130

pI: 5.70

mw: 79.08 kDa

protein length: 687 aa

gene length: 2064 bp

function: galactan utilization

product: beta-galactosidase

essential: no

ec: 3.2.1.23

synonyms: yvfN,lacA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.24|Utilization of galactan]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

Gene

Coordinates: 3,503,020 → 3,505,083

Phenotypes of a mutant

• a ''[gene|D4A5A878F87EBC14C02824233155CEBACEB41343|ganS]-[gene|75B1FD4AA7612B3B600CB0CE35319A6960536095|ganP]-[gene|676932E7DF143DC9AAAF1F415FB3D14EE25B2C2F|ganQ]-[gene|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA]-[gene|9DDEF5682B2D145830F33C7A1D5A6EC8527BB405|ganB]'' mutant is impaired in the utilization of galactan [Pubmed|22893383]

The protein

Catalyzed reaction/ biological activity

• degradation of galactotriose to galactose [Pubmed|22893383]
• degradation of ß-1.4-galactobiose to galactose [Pubmed|28617843]
• Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides (according to UniProt)

Protein family

• glycosyl hydrolase 42 family (with [protein|3226901F1D7C1F21296AB974CAE3656C18BE8F69|YesZ], according to UniProt)

Modification

• phosphorylated on Arg-387 [Pubmed|22517742]

Effectors of protein activity

• inhibited by galactose [pubmed|28617843]

Structure

• [PDB|4OJY] (from Geobacillus stearothermophilus, 76% identity) [pubmed|24100561]

Expression and Regulation

Operons

• genes: [gene|D4A5A878F87EBC14C02824233155CEBACEB41343|ganS]-[gene|75B1FD4AA7612B3B600CB0CE35319A6960536095|ganP]-[gene|676932E7DF143DC9AAAF1F415FB3D14EE25B2C2F|ganQ]-[gene|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA]-[gene|9DDEF5682B2D145830F33C7A1D5A6EC8527BB405|ganB]
  description: [Pubmed|9287030]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|27501980], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|1F787F70C87DEB221709579122D63C2322A84E56|GanR]: repression, [Pubmed|9287030], in [regulon|1F787F70C87DEB221709579122D63C2322A84E56|GanR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [pubmed|28617843], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • induced by galactan (binding of galactobiose to [protein|1F787F70C87DEB221709579122D63C2322A84E56|GanR]) [Pubmed|28617843,27501980]
  • repressed by glucose ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [Pubmed|28617843,27501980]

Biological materials

Mutant

• GP1181 (aphA3) available in [SW|Jörg Stülke]'s lab
• GP2147 (phleo) available in [SW|Jörg Stülke]'s lab
• 1A785 ( ''ganA''::''spec''), [Pubmed|11274134], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A785&Search=1A785 BGSC]
• 1A786 ( ''ganA''::''spec''), [Pubmed|11274134], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A786&Search=1A786 BGSC]
• BKE34130 (Δ[gene|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACATTCTCCTCCTTGT, downstream forward: _UP4_TAGGCTGATGCTCCGCTCGA
• BKK34130 (Δ[gene|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACATTCTCCTCCTTGT, downstream forward: _UP4_TAGGCTGATGCTCCGCTCGA

References

• 2104611,9287030,17056685,22517742,22893383,28617843,27501980,24100561