spoIVB

serine protease (site 1 protease), cleaves SpoIVFA to prepare it for CtpB cleavage, this results in pro-SigK processing/activation in the mother-cell, does also cleave CtpB

Locus: BSU_24230

Isoelectric point: 8.84

Molecular weight: 45.81 kDa

Protein length: 426 aa

Gene length: 1281 bp

Function: control of SigK activation

Product: serine protease

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → Sigma factors and their control → Control of sigma factors
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

Gene

Coordinates: 2,519,102 → 2,520,382

The protein

Catalyzed reaction/ biological activity

• Self-cleaves 52-Val-|-Asn-53, 62-Ala-|-Phe-63 and 74-Val-|-Thr-75 at the N-terminus of SpoIVB (according to UniProt)

Protein family

• PDZ protease PubMed

Domains

• PDZ domain (aa 101 - 187) (according to UniProt)
• Peptidase S55 domain (aa 188-426) (according to UniProt)

Localization

• secreted from the forespore to the intracellular space between the mother cell and the forespore PubMed

Expression and Regulation

Operons

• Genes: spoIVB
  Description: PubMed

  Sigma factors

  • SigF: sigma factor, PubMed, in SigF regulon
  • SigG: sigma factor, PubMed, in SigG regulon

  Regulatory mechanism

  • SpoVT: activation, PubMed, in SpoVT regulon

  Regulation

  • expressed during sporulation in the forespore (SigF, SigG, SpoVT) PubMed

Biological materials

Mutant

• BKE24230 (ΔspoIVB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCACTACTTCACTCTCC, downstream forward: _UP4_TGACTGCCGGAGTTTCCGGC
• BKK24230 (ΔspoIVB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCACTACTTCACTCTCC, downstream forward: _UP4_TGACTGCCGGAGTTTCCGGC

Labs working on this gene/protein

• Simon Cutting, London, UK homepage

References

Reviews

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Original Publications

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