epsF

similar to glycosyltransferase

Locus: BSU_34320

Isoelectric point: 6.93

Molecular weight: 42.46 kDa

Protein length: 384 aa

Gene length: 1155 bp

Function: biofilm formation

Product: unknown

Essential: no

Synonyms: yveP

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Matrix polysaccharide synthesis

Gene

Coordinates: 3,523,270 → 3,524,424

The protein

Protein family

• glycosyltransferase 1 family (according to UniProt)
• Glycosyltransferase 4 subfamily (according to UniProt)

Expression and Regulation

Operons

• Genes: epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • RemA: activation, PubMed, in RemA regulon
  • AbrB: repression, PubMed, in AbrB regulon
  • SinR: anti-activation, (prevents binding of RemA) PubMed, in SinR regulon
  • EAR riboswitch: processive antitermination, in EAR riboswitch

  Regulation

  • repressed by SinR PubMed

  Additional information

  • induction by sequestration of SinR by SinI or SlrA PubMed
  • expression is increased in rsiX mutants PubMed

Biological materials

Mutant

• MGNA-A069 (yveP::erm), available at the NBRP B. subtilis, Japan
• BKE34320 (ΔepsF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AACATGGAGCACGCGCTTTT, downstream forward: _UP4_AGCACGGAAAAGGACCATAA
• BKK34320 (ΔepsF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AACATGGAGCACGCGCTTTT, downstream forward: _UP4_AGCACGGAAAAGGACCATAA

Labs working on this gene/protein

• Richard Losick, Harvard Univ., Cambridge, USA homepage

References

Reviews

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The EAR RNA switch

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