SubtiBank SubtiBank
gcvPA
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gcvPA

glycine decarboxylase (subunit 1)
Locus
BSU_24560
Isoelectric point
6.95
Molecular weight
49.33 kDa
Protein length
448 aa Sequence Blast
Gene length
1347 bp Sequence Blast
Function
glycine utilization
Product
glycine decarboxylase (subunit 1)
Essential
no
E.C.
1.4.4.2
Synonyms
yqhJ,gcvP

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,546,869 → 2,548,215

The protein

Catalyzed reaction/ biological activity

  • glycine + H+ + N6-lipoyl-L-lysyl-[glycine-cleavage complex H protein] --> (R)-N6-(S8-aminomethyldihydrolipoyl)-L-lysyl-[glycine-cleavage complex H protein] + CO2 (according to UniProt)
  • Protein family

  • GcvP family (with GcvPB, according to UniProt)
  • Structure

  • 1WYT (the GcvPA-GcvPB complex from Thermus thermophilus, 56% identity) PubMed
  • Expression and Regulation

    Operons

    Description

    Regulatory mechanism

  • Gly-box: termination, PubMed, in Gly-box
  • Regulation

  • induced by glycine PubMed
  • the Gly-box RNA is degraded by RNase Y PubMed
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    Biological materials

    Mutant

  • MGNA-C415 (yqhJ::erm), available at the NBRP B. subtilis, Japan
  • BKE24560 (ΔgcvPA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGTTGTCTCCTCTCTC, downstream forward: _UP4_CTCATTCAGGAATTGGGGGA
  • BKK24560 (ΔgcvPA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGTTGTCTCCTCTCTC, downstream forward: _UP4_CTCATTCAGGAATTGGGGGA
  • References

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