gcvPA

glycine decarboxylase (subunit 1)

locus: BSU_24560

pI: 6.95

mw: 49.33 kDa

protein length: 448 aa

gene length: 1347 bp

function: glycine utilization

product: glycine decarboxylase (subunit 1)

essential: no

ec: 1.4.4.2

synonyms: yqhJ,gcvP

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.8|Utilization of threonine/ glycine]

Gene

Coordinates: 2,546,869 → 2,548,215

The protein

Catalyzed reaction/ biological activity

• glycine + H⁺ + N⁶-lipoyl-L-lysyl-[glycine-cleavage complex H protein] --> (R)-N⁶-(S⁸-aminomethyldihydrolipoyl)-L-lysyl-[glycine-cleavage complex H protein] + CO₂ (according to UniProt)

Protein family

• GcvP family (with [protein|20175DF9FCF07C34A05C572B089C33D1E1FE6DF2|GcvPB], according to UniProt)

Structure

• [PDB|1WYT] (the [protein|DB0618EBA12013842ED3972165D0276F438D8EC1|GcvPA]-[protein|20175DF9FCF07C34A05C572B089C33D1E1FE6DF2|GcvPB] complex from Thermus thermophilus, 56% identity) [pubmed|15791207]

Expression and Regulation

Operons

• genes: [gene|F6F449170276055E6D60D89E9E17A152A82CA166|gcvT]-[gene|DB0618EBA12013842ED3972165D0276F438D8EC1|gcvPA]-[gene|20175DF9FCF07C34A05C572B089C33D1E1FE6DF2|gcvPB]
  description: [Pubmed|15472076]

  regulatory mechanism

  • [regulon|Gly-box|Gly-box]: termination, [pubmed|31992591], in [regulon|Gly-box|Gly-box]

  regulation

  • induced by glycine [Pubmed|15472076]
  • the [SW|Gly-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

Biological materials

Mutant

• MGNA-C415 (yqhJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2413 NBRP B. subtilis, Japan]
• BKE24560 (Δ[gene|DB0618EBA12013842ED3972165D0276F438D8EC1|gcvPA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGTTGTCTCCTCTCTC, downstream forward: _UP4_CTCATTCAGGAATTGGGGGA
• BKK24560 (Δ[gene|DB0618EBA12013842ED3972165D0276F438D8EC1|gcvPA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGTTGTCTCCTCTCTC, downstream forward: _UP4_CTCATTCAGGAATTGGGGGA

References

• 12107147,15472076,15791207