mtbP [2019-07-01 09:25:55]

DNA (cytosine-5-)-methyltransferase

locus: BSU20250

pI: 7.80

mw: 50.35 kDa

protein length: 443 aa

gene length: 1332 bp

function: DNA modification

product: DNA (cytosine-5-)-methyltransferase

essential: no

ec: 2.1.1.37

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.4|DNA restriction/ modification]
• [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.2|SP-beta prophage]

Gene

Coordinates: 2,171,401 → 2,172,732

The protein

Catalyzed reaction/ biological activity

• S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine (according to Swiss-Prot)

Protein family

• [SW|Methyltransferase superfamily] (according to UniProt)
• C5-methyltransferase family (with [protein|CEA337DD4C7DFC22247FDA7FA1E71356FB819C77|YdiP] and [protein|9EE25F97B2C6240E31BA1D5EEE9DA2D7940C9637|YdiO], according to UniProt)

Structure

• [PDB|1MHT] (from Haemophilus haemolyticus, 28% identity) [pubmed|8293469]

Biological materials

Mutant

• BKE20250 (Δ[gene|DAE0C61FD9902AD3092A779105B4A0E656069266|mtbP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE20250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACAAACAACCTCCATATA, downstream forward: _UP4_TAAAATTTGTCTTTTAAACA
• BKK20250 (Δ[gene|DAE0C61FD9902AD3092A779105B4A0E656069266|mtbP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK20250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACAAACAACCTCCATATA, downstream forward: _UP4_TAAAATTTGTCTTTTAAACA

References

Research papers

• 8293469