SubtiBank SubtiBank
mtbP
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mtbP

DNA (cytosine-5-)-methyltransferase
Locus
BSU_20250
Isoelectric point
7.80
Molecular weight
50.35 kDa
Protein length
443 aa Sequence Blast
Gene length
1332 bp Sequence Blast
Function
DNA modification
Product
DNA (cytosine-5-)-methyltransferase
Essential
no
E.C.
2.1.1.37
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Genetics → DNA restriction/ modification
  • Prophages and mobile genetic elements → Prophages → SP-beta prophage

    • Gene

    Coordinates
    2,171,401 → 2,172,732

    The protein

    Catalyzed reaction/ biological activity

  • 2'-deoxycytidine in DNA + S-adenosyl-L-methionine --> 5-methyl-2'-deoxycytidine in DNA + H+ + S-adenosyl-L-homocysteine (according to UniProt)

    • Protein family

  • Methyltransferase superfamily (according to UniProt)
  • class I-like SAM-binding methyltransferase superfamily (according to UniProt)

    • Domains

  • SAM-dependent MTase C5-type domain (aa 4-440) (according to UniProt)

    • Structure

  • 1MHT (from Haemophilus haemolyticus, 28% identity) PubMed

    • Biological materials

    Mutant

  • BKE20250 (ΔmtbP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACAAACAACCTCCATATA, downstream forward: _UP4_TAAAATTTGTCTTTTAAACA
  • BKK20250 (ΔmtbP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACAAACAACCTCCATATA, downstream forward: _UP4_TAAAATTTGTCTTTTAAACA

    • References

    Research papers

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