ackA [2019-08-08 13:32:17]

acetate kinase

Locus: BSU_29470

Isoelectric point: 5.19

Molecular weight: 42.98 kDa

Protein length: 395 aa

Gene length: 1188 bp

Function: overflow metabolism

Product: acetate kinase

Essential: no

E.C.: 2.7.2.1

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Electron transport and ATP synthesis → ATP synthesis → Substrate-level phosphorylation
• Metabolism → Carbon metabolism → Carbon core metabolism → Overflow metabolism

Gene

Coordinates: 3,015,111 → 3,016,298

The protein

Catalyzed reaction/ biological activity

• ATP + acetate = ADP + acetyl phosphate (according to Swiss-Prot)

Protein family

• acetokinase family (with Buk, according to UniProt)

Structure

• 2IIR (from Thermotoga maritima, 54% identity, 73% similarity)

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: ackA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CodY: activation, PubMed, in CodY regulon
  • CcpA: activation, PubMed, in CcpA regulon

  Regulation

  • activated during growth in the presence of branched chain amino acids (CodY) PubMed

Biological materials

Mutant

• GP1902 (ackA::aphA3) (available in Jörg Stülke's lab)
• BKE29470 (ΔackA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATTGACGCTCCTTTAT, downstream forward: _UP4_TAAATCGCATGAAAGCACAT
• BKK29470 (ΔackA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATTGACGCTCCTTTAT, downstream forward: _UP4_TAAATCGCATGAAAGCACAT

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

Labs working on this gene/protein

• Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

References

Reviews

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Original publications

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