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ackA [Sun Nov 27 2016 17:36:37 GMT+0100 (CET)]
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ackA [Sun Nov 27 2016 17:36:37 GMT+0100 (CET)]

acetate kinase
locus
BSU29470
pI
5.00
mw
42.00 kDa
function
overflow metabolism
product
acetate kinase
essential
no
ec
2.7.2.1
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.5|ATP synthesis] → [category|SW 2.1.5.2|Substrate-level phosphorylation]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.5|Overflow metabolism]

    • [SW|Categories] containing this gene/protein

  • [SW|ATP synthesis], [SW|carbon core metabolism], [SW|most abundant proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|CcpA regulon], [SW|CodY regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    3,015,111 -> 3,016,298

    The protein

    Catalyzed reaction/ biological activity

  • ATP acetate = ADP acetyl phosphate (according to Swiss-Prot)

    • Protein family

  • acetokinase family (according to Swiss-Prot)

    • Structure

  • [PDB|2IIR] (from ''Thermotoga maritima'', 54% identity, 73% similarity)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operon

  • ''[protein|search|ackA]'' [Pubmed|8226682]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|8226682]

    • Regulation

  • expression activated by glucose (2.5 fold) ([protein|search|CcpA]) [Pubmed|8226682,12850135]
  • activated during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|16995897]

    • Regulatory mechanism

  • [protein|search|CodY]: transcription activation [Pubmed|16995897]
  • [protein|search|CcpA]: transcription activation [Pubmed|8226682,12850135]

    • Additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Biological materials

    Mutant

  • GP1902 (''ackA''::''aphA3'') (available in [SW|Jörg Stülke]'s lab)

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]

    • References

    Reviews

  • 16678422,15755952

    • Original publications

  • 8226682,9811655,15378759,11244084,16995897,12850135,8226682,9811655,21106498