gerAC

lipoprotein, nutrient receptor, germination response to L-alanine, triggers premature germination in response to morphological defects during sporulation

Locus: BSU_33070

Isoelectric point: 7.30

Molecular weight: 42.23 kDa

Protein length: 373 aa

Gene length: 1122 bp

Function: germination response to L-alanine

Product: nutrient receptor

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Sporulation → Germination → Germinant receptors
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,393,293 → 3,394,414

Phenotypes of a mutant

• suppresses the defects of spoVV mutant spores PubMed

The protein

Catalyzed reaction/ biological activity

• triggers premature germination in response to morphological defects during sporulation PubMed

Protein family

• GerABKC lipoprotein family (according to UniProt)

Paralogous protein(s)

• GerBC

Domains

• has an N-terminal signal sequence, lipidated on a Cys residue adjacent to the signal peptide PubMed

Structure

• 3N54 (GerBC, 34% identity) PubMed

Localization

• cell membrane (according to UniProt)
• outer surface of the inner spore membrane PubMed

Additional information

• 1,100 molecules are present per spore PubMed

Expression and Regulation

Operons

• Genes: gerAA-gerAB-gerAC
  Description: PubMed

  Sigma factors

  • SigG: sigma factor, PubMed, in SigG regulon
  • SigF: sigma factor, PubMed, in SigF regulon

  Regulatory mechanism

  • SpoVT: repression, PubMed, in SpoVT regulon

  Regulation

  • expressed during sporulation in the forespore (SigF, SigG, SpoVT) PubMed

  Additional information

  • 1,100 molecules are present per spore PubMed

Biological materials

Mutant

• BKE33070 (ΔgerAC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAACATACATAAAATCCGGA, downstream forward: _UP4_AAAGGCGCAAACAAATAGCA
• BKK33070 (ΔgerAC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAACATACATAAAATCCGGA, downstream forward: _UP4_AAAGGCGCAAACAAATAGCA

References

Reviews

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Original publications

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