gmuD

phospho-beta-mannosidase

locus: BSU_05840

pI: 5.73

mw: 54.17 kDa

protein length: 465 aa

gene length: 1398 bp

function: glucomannan utilization

product: phospho-beta-mannosidase

essential: no

synonyms: ydhP

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.25|Utilization of glucomannan]

Gene

Coordinates: 628,630 → 630,027

The protein

Catalyzed reaction/ biological activity

• 6-phospho-β-D-glucosyl-(1→4)-D-glucose + H₂O --> D-glucose + D-glucose 6-phosphate (according to UniProt)

Protein family

• [SW|glycosyl hydrolase 1 family] (according to UniProt)

Structure

• [PDB|4B3K] (from Streptococcus pyogenes, 50% identity) [pubmed|23275159]

Expression and Regulation

Operons

• genes: [gene|00A4A90685CED4AD46829C45718CF26F1D62F5D3|gmuB]-[gene|44014896157718D3A6190DFDBD663C5CB9DA63FC|gmuA]-[gene|76324E0A6CAA8E1DD0B0C6FAE1CAB402231BF7CC|gmuC]-[gene|D8C81A8768E9C6B47B5E196B54DC2A3A8256E7E6|gmuD]-[gene|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|gmuR]-[gene|EB6DE119E6FB18413DB2C2B62B114FB787164F74|gmuE]-[gene|35225776F6044513D3E77DBBD6A7A8FE976F506A|gmuF]-[gene|289ACC9D13BBFAA5C083EE525ECCC32DB06E075F|gmuG]
  description: [Pubmed|18177310]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|18177310], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|GmuR]: repression, [Pubmed|18177310], in [regulon|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|GmuR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|18177310], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: activation, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • induced by cellobiose ([protein|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|GmuR]) [Pubmed|18177310]

Biological materials

Mutant

• MGNA-C191 (ydhP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2189 NBRP B. subtilis, Japan]
• BKE05840 (Δ[gene|D8C81A8768E9C6B47B5E196B54DC2A3A8256E7E6|gmuD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGCCAAGTCTCTCTCCCCTT, downstream forward: _UP4_TAGATTTTCCTAAAGGTCAA
• BKK05840 (Δ[gene|D8C81A8768E9C6B47B5E196B54DC2A3A8256E7E6|gmuD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGCCAAGTCTCTCTCCCCTT, downstream forward: _UP4_TAGATTTTCCTAAAGGTCAA

References

• 14652714,18177310,15139916,10627040,20817675,23275159