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ycsE
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ycsE

5'-nucleotidase, dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate
locus
BSU_04040
pI
4.49
mw
27.92 kDa
protein length
249 aa Sequence Blast
gene length
750 bp Sequence Blast
function
dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate
product
5'-nucleotidase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.2|Biosynthesis/ acquisition of riboflavin/ FAD]

    • Gene

    Coordinates
    456,068 → 456,817

    The protein

    Catalyzed reaction/ biological activity

  • dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate [Pubmed|26316208]
  • 5-amino-6-(5-phospho-D-ribitylamino)uracil + H2O --> 5-amino-6-(D-ribitylamino)uracil + phosphate (according to UniProt)

    • Protein family

  • [SW|HAD superfamily] (according to UniProt) [Pubmed|26316208]
  • [SW|Cof family] (according to UniProt)

    • Structure

  • [PDB|1NRW] ([protein|AF9523D3AA3C032B70175BCFFED34E9A42ED01AF|PhoC], 31% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|D8AC0ECC57C6113D0CCA36FC38395EECBD00FF50|ycsE]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-C071 (ycsE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2069 NBRP B. subtilis, Japan]
  • BKE04040 (Δ[gene|D8AC0ECC57C6113D0CCA36FC38395EECBD00FF50|ycsE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATGTACCTCTCTTTA, downstream forward: _UP4_TAAAAAAAGAGAGTCCTAAG
  • BKK04040 (Δ[gene|D8AC0ECC57C6113D0CCA36FC38395EECBD00FF50|ycsE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATGTACCTCTCTTTA, downstream forward: _UP4_TAAAAAAAGAGAGTCCTAAG

    • References

  • 16990279,27784292,26316208,22383849,25848029