khtT

K⁺/H⁺ antiporter for K⁺ efflux

Locus: BSU_09860

Isoelectric point: 4.89

Molecular weight: 18.59 kDa

Protein length: 165 aa

Gene length: 498 bp

Function: potassium ion efflux

Product: K⁺/H⁺ antiporter for K⁺ efflux

Essential: no

Synonyms: yhaT

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Metal ion transporter
• Cellular processes → Homeostasis → Metal ion homeostasis (K, Na, Ca, Mg) → Potassium uptake/ export
• Information processing → Targets of second messengers → Targets of c-di-AMP
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,060,427 → 1,060,924

The protein

Domains

• contains a RCK_C domain at the C-terminus (according to UniProt, x)

Effectors of protein activity

• binds c-di-AMP, which likely activates export activity PubMed

Localization

• cell membrane (peripheral) (according to UniProt)

Expression and Regulation

Operons

• Genes: khtS-khtT-khtU
  Description: PubMed

Biological materials

Mutant

• MGNA-A675 (yhaT::erm), available at the NBRP B. subtilis, Japan
• GP2078 (ΔkhtS-khtT-khtU::aphA3), available in Jörg Stülke's lab
• BKE09860 (ΔkhtT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATGAAAATCCCTCCGAAT, downstream forward: _UP4_TCTGGAGAGGGCGTGTGAGC
• BKK09860 (ΔkhtT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATGAAAATCCCTCCGAAT, downstream forward: _UP4_TCTGGAGAGGGCGTGTGAGC

Expression vectors

• pGP2906 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab

References

Reviews

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Original publications

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