yqcG

toxin, eliminates defective cells from developing biofilms, DNase activity

Locus: BSU_25860

Isoelectric point: 8.81

Molecular weight: 59.50 kDa

Protein length: 531 aa

Gene length: 1596 bp

Function: eliminates defective cells from developing biofilms

Product: toxin

Essential: yes

Synonyms

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Coping with stress → Toxins, antitoxins and immunity against toxins → Type 2 TA systems
• Lifestyles → Coping with stress → Toxins, antitoxins and immunity against toxins/ based on similarity
• Prophages and mobile genetic elements → Prophages → Skin element

Gene

Coordinates: 2,661,102 → 2,662,697

The protein

Catalyzed reaction/ biological activity

• part of a type II toxin/antitoxin system (with YqcF) PubMed
• eliminates defective cells from developing biofilms PubMed
• DNA degradation PubMed

Domains

• LXG domain (aa 1-235) (according to UniProt)

Expression and Regulation

Operons

• Genes: yqcG-yqcF
  Description: PubMed

  Regulatory mechanism

  • Spo0A: activation, PubMed, in Spo0A regulon
  • AbrB: repression, PubMed, in AbrB regulon

  Regulation

  • expressed under conditions that trigger sporulation (Spo0A) PubMed

Biological materials

Mutant

• BKE25860 (ΔyqcG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATCATATCCTTTCATT, downstream forward: _UP4_GGAGATTAAGGAGAGATTGT
• BKK25860 (ΔyqcG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATCATATCCTTTCATT, downstream forward: _UP4_GGAGATTAAGGAGAGATTGT

References

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