yugK

similar to NADH-dependent butanol dehydrogenase

locus: BSU_31360

pI: 4.64

mw: 43.25 kDa

protein length: 390 aa

gene length: 1173 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 3,222,195 → 3,223,367

The protein

Protein family

• iron-containing alcohol dehydrogenase family (with [protein|1E951C2B640966643D5F081159522CD1C51942C8|YugJ] and [protein|B90D4E798145F2AF3421DDC5AB6CD578E2E17900|GbsB], according to UniProt)

Paralogous protein(s)

• [protein|1E951C2B640966643D5F081159522CD1C51942C8|YugJ]

Structure

• [PDB|1VLJ] (from Thermotoga Maritima 42% identity)

Expression and Regulation

Operons

• genes: [gene|D81610C7CA2A92FCB1195591ACB613E7C1288F6B|yugK]
  description: [Pubmed|9274030]

Biological materials

Mutant

• MGNA-B548 (yugK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1547 NBRP B. subtilis, Japan]
• BKE31360 (Δ[gene|D81610C7CA2A92FCB1195591ACB613E7C1288F6B|yugK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGTAAAACCTCCAAATG, downstream forward: _UP4_TAAACCCAAAGGGCGGAATC
• BKK31360 (Δ[gene|D81610C7CA2A92FCB1195591ACB613E7C1288F6B|yugK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGTAAAACCTCCAAATG, downstream forward: _UP4_TAAACCCAAAGGGCGGAATC

References

Reviews

• 20924577

Original publications

• 9274030

The corresponding protein in ''E. coli

• 20543070,18211903