azlB

transcriptional repressor ([SW|Lrp family]) of the [gene|D7397591C2157EBB1951ED3DC1E315F9AE7A4BB3|azlB]-[gene|C90D642197EE589F9D33CD5AA0B1069E3BC124A8|azlC]-[gene|7A1445B7AFD9C3AA45EF67E51F5D233FCC5F8884|azlD]-[gene|8BB8BDA602CC26B0A9E8C22666F0EC539306CF39|brnQ]-[gene|17FFEA4977F4F7334CFF7CD1C644DF14F599E6E6|yrdK] operon

locus: BSU_26720

pI: 4.87

mw: 17.32 kDa

protein length: 157 aa

gene length: 474 bp

function: regulation of branched-chain amino acid transport

product: transcriptional repressor ([SW|Lrp family])

essential: no

synonyms: yrdG

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.12|Biosynthesis/ acquisition of branched-chain amino acids]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.9|Utilization of branched-chain amino acids]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 2,729,753 → 2,730,226

The protein

Protein family

• [SW|Lrp family]

[SW|Domains]

• [SW|HTH asnC-type domain] (aa 5-66) (according to UniProt)

Structure

• [PDB|2GQQ] (E. coli Lrp, 34% identity) [pubmed|17223133]

Expression and Regulation

Operons

• genes: [gene|D7397591C2157EBB1951ED3DC1E315F9AE7A4BB3|azlB]-[gene|C90D642197EE589F9D33CD5AA0B1069E3BC124A8|azlC]-[gene|7A1445B7AFD9C3AA45EF67E51F5D233FCC5F8884|azlD]-[gene|8BB8BDA602CC26B0A9E8C22666F0EC539306CF39|brnQ]-[gene|17FFEA4977F4F7334CFF7CD1C644DF14F599E6E6|yrdK]
  description: [Pubmed|9287000]

  regulatory mechanism

  • [protein|D7397591C2157EBB1951ED3DC1E315F9AE7A4BB3|AzlB]: repression, [Pubmed|9287000], in [regulon|D7397591C2157EBB1951ED3DC1E315F9AE7A4BB3|AzlB regulon]

  regulation

  • repressed by [protein|search|AzlB] [Pubmed|9287000]

Biological materials

Mutant

• BKE26720 (Δ[gene|D7397591C2157EBB1951ED3DC1E315F9AE7A4BB3|azlB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE26720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCACAACCCCCAATAC, downstream forward: _UP4_GATGAAATGTAGGAGGTTGT
• BKK26720 (Δ[gene|D7397591C2157EBB1951ED3DC1E315F9AE7A4BB3|azlB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK26720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCACAACCCCCAATAC, downstream forward: _UP4_GATGAAATGTAGGAGGTTGT

References

• 9287000,17223133