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sucC [Wed Aug 24 2016 13:24:42 GMT+0200 (CEST)]
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sucC [Wed Aug 24 2016 13:24:42 GMT+0200 (CEST)]

succinyl-CoA synthetase (beta subunit)
locus
BSU16090
pI
4.00
mw
41.00 kDa
function
TCA cycle
product
succinyl-CoA synthetase (beta subunit)
essential
no
ec
6.2.1.5
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.5|ATP synthesis] → [category|SW 2.1.5.2|Substrate-level phosphorylation]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

    • [SW|Categories] containing this gene/protein

  • [SW|ATP synthesis], [SW|carbon core metabolism], [SW|phosphoproteins], [SW|most abundant proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|CcpA regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    1,680,431 -> 1,681,588

    The protein

    Catalyzed reaction/ biological activity

  • ATP succinate CoA = ADP phosphate succinyl-CoA (according to Swiss-Prot)

    • Protein family

  • ATP-grasp domain (according to Swiss-Prot)

    • Kinetic information

  • Reversible Michaelis-Menten [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-44C8RWC-SH&_user=5731894&_coverDate=01/01/1985&_rdoc=28&_fmt=high&_orig=browse&_srch=doc-info(#toc#4938#1985#998209998#270526#FLP#display#Volume)&_cdi=4938&_sort=d&_docanchor=&_ct=40&_acct=C000043105&_version=1&_urlVersion=0&_userid=5731894&md5=f14f4734123ab1177d7217cab6c7ce7d FEBS Letters]

    • Modification

  • phosphorylation on Ser-220 [Pubmed|17218307]

    • Effectors of protein activity

  • Inhibited by 2-oxoglutarate, ATP and NADH [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-44C8RWC-SH&_user=5731894&_coverDate=01/01/1985&_rdoc=28&_fmt=high&_orig=browse&_srch=doc-info(#toc#4938#1985#998209998#270526#FLP#display#Volume)&_cdi=4938&_sort=d&_docanchor=&_ct=40&_acct=C000043105&_version=1&_urlVersion=0&_userid=5731894&md5=f14f4734123ab1177d7217cab6c7ce7d FEBS Letters]
  • GTP is not accepted by the enzyme [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-44C8RWC-SH&_user=5731894&_coverDate=01/01/1985&_rdoc=28&_fmt=high&_orig=browse&_srch=doc-info(#toc#4938#1985#998209998#270526#FLP#display#Volume)&_cdi=4938&_sort=d&_docanchor=&_ct=40&_acct=C000043105&_version=1&_urlVersion=0&_userid=5731894&md5=f14f4734123ab1177d7217cab6c7ce7d FEBS Letters]

    • Structure

  • [PDB|1JKJ] (E. coli)

    • [SW|Interactions]

  • [protein|search|Mdh]-[protein|search|SucC] [Pubmed|20933603]
  • [protein|search|SucC]-[protein|search|SucD] [Pubmed|20933603]

    • Additional information

  • extensive information on the structure and enzymatic properties of succinyl-CoA synthetase can be found at [http://www.proteopedia.org/wiki/index.php/Succinyl-Coa_Synthetase Proteopedia]

    • Expression and Regulation

    Operon

  • ''[protein|search|sucC]''-''[protein|search|sucD]'' [Pubmed|11976317]

    • Regulation

  • repressed by glucose (2.7-fold) ([protein|search|CcpA]) [Pubmed|12850135]

    • Regulatory mechanism

  • [protein|search|CcpA]: transcription repression [Pubmed|12850135]
  • [protein|search|RoxS]: inhibition of translation and initiation of RNA degradation by [rnc|search|RNase III] [Pubmed|25643072]

    • Additional information

  • the mRNA base-pairs with the [protein|search|RoxS] [protein|search|sRNA] [PubMed|25643072]
  • the '[protein|search|roxS]-[protein|search|sucC]' RNA duplex forms a cleavage site for [rnc|search|RNase III] [PubMed|25643072]
  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Biological materials

    Mutant

  • 1A1006 ( ''sucC''::''spec''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1006&Search=1A1006 BGSC]
  • GP1134 (cat), available in [SW|Jörg Stülke]'s lab
  • GP791 (''[protein|search|sucC]-[protein|search|sucD]''::''tet''), available in [SW|Jörg Stülke]'s lab
  • GP2344 (''[protein|search|sucC]-[protein|search|sucD]''::''kan''), Cre-recombinase is integrated in ''[protein|search|sacA]'', available in [SW|Jörg Stülke]'s lab
  • GP2345 (''[protein|search|sucC]-[protein|search|sucD]''::''lox72''), Cre-recombinase is integrated in ''[protein|search|sacA]'', available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • References

  • 12850135,17218307,11976317,20933603,15378759,22900538,25643072