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sucC [2019-08-01 11:16:38]
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sucC [2019-08-01 11:16:38]

succinyl-CoA synthetase (beta subunit)
Locus
BSU_16090
Isoelectric point
4.85
Molecular weight
41.21 kDa
Protein length
385 aa Sequence Blast
Gene length
1158 bp Sequence Blast
Function
TCA cycle
Product
succinyl-CoA synthetase (beta subunit)
Essential
no
E.C.
6.2.1.5
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,680,431 → 1,681,588

The protein

Catalyzed reaction/ biological activity

  • ATP succinate CoA = ADP phosphate succinyl-CoA (according to Swiss-Prot)
  • Protein family

  • succinate/malate CoA ligase beta subunit family (single member, according to UniProt)
  • Kinetic information

  • Reversible Michaelis-Menten FEBS Letters
  • Modification

  • phosphorylation on Ser-220 PubMed
  • Effectors of protein activity

  • Inhibited by 2-oxoglutarate, ATP and NADH FEBS Letters
  • GTP is not accepted by the enzyme FEBS Letters
  • Structure

  • 1JKJ (E. coli)
  • Additional information

  • extensive information on the structure and enzymatic properties of succinyl-CoA synthetase can be found at Proteopedia
  • belongs to the 100 most abundant proteins PubMed
  • Expression and Regulation

    Operons

    Description

    Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • Regulation

  • repressed by glucose (2.4-fold) (CcpA) PubMed
  • expression is heterogeneous PubMed
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    Other regulations

  • RoxS: translation inhibition, translation inhibition and initiation of RNA degradation by Rnc
  • Biological materials

    Mutant

  • 1A1006 ∆( sucC)::spec, available at BGSC
  • GP1134 (cat), available in Jörg Stülke's lab
  • GP791 ∆(sucC-sucD)::tet, available in Jörg Stülke's lab
  • GP2344 ∆(sucC-sucD)::kan, Cre-recombinase is integrated in sacA, available in Jörg Stülke's lab
  • GP2345 ∆(sucC-sucD::lox72), Cre-recombinase is integrated in sacA, available in Jörg Stülke's lab
  • BKE16090 Δ(sucC)::erm trpC2 available at BGSC, PubMed, upstream reverse: _UP1_CATTTCCCATCCTCCTAACT, downstream forward: _UP4_TAAGAAAGAATGAAAGGCAG
  • BKK16090 Δ(sucC)::kan trpC2 available at BGSC, PubMed, upstream reverse: _UP1_CATTTCCCATCCTCCTAACT, downstream forward: _UP4_TAAGAAAGAATGAAAGGCAG
  • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • References

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