pyrAB

carbamoyl-phosphate synthetase (catalytic subunit)

locus: BSU_15520

pI: 4.78

mw: 117.44 kDa

protein length: 1071 aa

gene length: 3216 bp

function: pyrimidine biosynthesis

product: carbamoyl-phosphate synthetase (catalytic subunit)

essential: no

ec: 6.3.5.5

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.4|Biosynthesis/ acquisition of pyrimidine nucleotides]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,623,736 → 1,626,951

The protein

Catalyzed reaction/ biological activity

• 2 ATP + H₂O + hydrogencarbonate + L-glutamine --> 2 ADP + carbamoyl phosphate + 2 H⁺ + L-glutamate + phosphate (according to UniProt)

Protein family

• carB family (with [protein|7E6386F1949A6F46E4332BAB8F803742EB076AF0|CarB], according to UniProt)

Paralogous protein(s)

• [protein|7E6386F1949A6F46E4332BAB8F803742EB076AF0|CarB]

[SW|Domains]

• 2 [SW|ATP-grasp domain]s (aa 133-327, aa 671-861) (according to UniProt)
• MGS-like domain (aa 930-1071) (according to UniProt)

Structure

• [PDB|1JDB] (from ''Escherichia coli'', 49% identity, 69% similarity) [Pubmed|10089390]

[SW|Localization]

• membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]-[gene|A5C26E10085868A0A193C450DF58D54C636B7C2D|pyrP]-[gene|B83462A50FDDD20FE9BD4681824E30AAD10F506B|pyrB]-[gene|CA16FD3507DCCADB1E01DF25B42C3405C9205B05|pyrC]-[gene|E7741ED6F15044900BDC5F0584246469D2D4D586|pyrAA]-[gene|D57DC8A53E52BC4A2EA894E3C9FD983592882918|pyrAB]-[gene|C20A05AE4CE8AEE0DA767334935CC64106A7FCCB|pyrK]-[gene|E533BEF28579CD04B24E2C728737269E35224450|pyrD]-[gene|5D0D0D2413377FCA92440334E92B5A27A5963D9E|pyrF]-[gene|0197A037D2AF049B295E1DA60187D4614B2BAC51|pyrE]
  description: [Pubmed|8206849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1709162], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|PyrR]: termination/ antitermination, via [SW|RNA switch], in [regulon|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|PyrR regulon]

  regulation

  • induced in the absence of uridine nucleotides ([protein|search|PyrR]) [Pubmed|8206849]

Biological materials

Mutant

• BKE15520 (Δ[gene|D57DC8A53E52BC4A2EA894E3C9FD983592882918|pyrAB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATTACTAAAATTTTGTTAA, downstream forward: _UP4_AATCAGGAGGCGGCAGTCAC
• BKK15520 (Δ[gene|D57DC8A53E52BC4A2EA894E3C9FD983592882918|pyrAB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATTACTAAAATTTTGTTAA, downstream forward: _UP4_AATCAGGAGGCGGCAGTCAC

References

Reviews

• 12859215,11395405,11163353

Original publications

• 8206849,1709162,18763711,10089390,8663035