cotI

outer spore coat protein

Locus: BSU_30920

Isoelectric point: 4.99

Molecular weight: 41.09 kDa

Protein length: 357 aa

Gene length: 1074 bp

Function: spore envelope

Product: outer spore coat protein

Essential: no

Synonyms: ytaA

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Sporulation → Sporulation proteins → Spore coat proteins → Not yet assigned

Gene

Coordinates: 3,162,084 → 3,163,157

The protein

Protein family

• cotS family (with CotS and YutH, according to UniProt)

Structure

• 2Q83 PubMed

Localization

• outer spore coat PubMed

Expression and Regulation

Operons

• Genes: cotI
  Description: PubMed

  Sigma factors

  • SigK: sigma factor, PubMed, in SigK regulon

  Regulation

  • expressed late during sporulation in the mother cell (SigK) PubMed

Biological materials

Mutant

• MGNA-A030 (ytaA::erm), available at the NBRP B. subtilis, Japan
• BKE30920 (ΔcotI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCTTACCTGTTTTACTC, downstream forward: _UP4_TAAGACCATCATTTCCCCCG
• BKK30920 (ΔcotI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCTTACCTGTTTTACTC, downstream forward: _UP4_TAAGACCATCATTTCCCCCG

References

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