cwlH

N-acetylmuramoyl-L-alanine amidase

locus: BSU_25710

pI: 10.00

mw: 27.42 kDa

protein length: 250 aa

gene length: 753 bp

function: cell wall metabolism

product: N-acetylmuramoyl-L-alanine amidase

essential: no

ec: 3.5.1.28

synonyms: yqeE

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.1|Autolysis]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 2,648,903 → 2,649,655

The protein

Catalyzed reaction/ biological activity

• Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

Protein family

• [SW|N-acetylmuramoyl-L-alanine amidase 2 family] (according to UniProt)

Paralogous protein(s)

• [protein|50D7B97E4D3D41F74EF1942B85DE1DA4A33BEBB0|XlyA], [protein|A8AF827637B7208DFA101C9A2911BF9AB1AED08F|XlyB], [protein|C9C395A5F6A2C06EAC97F228C5C66D1628C937CF|CwlA]

[SW|Domains]

• contains an amidase_2 domain (like [protein|C1EB8D05386C1B35B8002239F5247CC6AB25F786|BlyA], [protein|C9C395A5F6A2C06EAC97F228C5C66D1628C937CF|CwlA], [protein|50D7B97E4D3D41F74EF1942B85DE1DA4A33BEBB0|XlyA], [protein|A8AF827637B7208DFA101C9A2911BF9AB1AED08F|XlyB])
• [SW|N-acetylmuramoyl-L-alanine amidase domain] (aa 45-141) (according to UniProt)

Structure

• [PDB|3RDR] (N-terminal domain, aa 1 - 150, XlyA, 75% identity) [pubmed|21816821]
• [PDB|1LBU] (C-terminal peptidoglycan-binding domain, aa 183 - 245, 48% identity)

[SW|Localization]

• secreted (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|D5612882F1887BE342EF7072E71502382AB7289F|cwlH]
  description: [Pubmed|10515909]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|10515909], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

  regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: activation, [Pubmed|10515909], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]

  regulation

  • expressed late during sporulation in the mother cell ([protein|search|SigK], [protein|search|GerE]) [Pubmed|10515909]

Biological materials

Mutant

• BKE25710 (Δ[gene|D5612882F1887BE342EF7072E71502382AB7289F|cwlH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTATCCCTCCCTATT, downstream forward: _UP4_TAAATGCAGGATTTAACGGA
• BKK25710 (Δ[gene|D5612882F1887BE342EF7072E71502382AB7289F|cwlH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTATCCCTCCCTATT, downstream forward: _UP4_TAAATGCAGGATTTAACGGA

References

• 10945275,10515909,21816821