cwlH

N-acetylmuramoyl-L-alanine amidase

Locus: BSU_25710

Isoelectric point: 10.00

Molecular weight: 27.42 kDa

Protein length: 250 aa

Gene length: 753 bp

Function: cell wall metabolism

Product: N-acetylmuramoyl-L-alanine amidase

Essential: no

E.C.: 3.5.1.28

Synonyms: yqeE

Genomic Context

Loading

Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Autolysis
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
• Groups of genes → Secreted proteins

Gene

Coordinates: 2,648,903 → 2,649,655

The protein

Catalyzed reaction/ biological activity

• Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

Protein family

• N-acetylmuramoyl-L-alanine amidase 2 family (according to UniProt)

Paralogous protein(s)

• XlyA, XlyB, CwlA

Domains

• contains an amidase_2 domain (like BlyA, CwlA, XlyA, XlyB)
• N-acetylmuramoyl-L-alanine amidase domain (aa 45-141) (according to UniProt)

Structure

• 3RDR (N-terminal domain, aa 1 - 150, XlyA, 75% identity) PubMed
• 1LBU (C-terminal peptidoglycan-binding domain, aa 183 - 245, 48% identity)

Localization

• secreted (according to UniProt)

Expression and Regulation

Operons

• Genes: cwlH
  Description: PubMed

  Sigma factors

  • SigK: sigma factor, PubMed, in SigK regulon

  Regulatory mechanism

  • GerE: activation, PubMed, in GerE regulon

  Regulation

  • expressed late during sporulation in the mother cell (SigK, GerE) PubMed

Biological materials

Mutant

• BKE25710 (ΔcwlH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCTATCCCTCCCTATT, downstream forward: _UP4_TAAATGCAGGATTTAACGGA
• BKK25710 (ΔcwlH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCTATCCCTCCCTATT, downstream forward: _UP4_TAAATGCAGGATTTAACGGA

References

Loading