5'-3' double-stranded DNA exonuclease, coordination of cell division with DNA replication
function
coordination of cell division with DNA replication
product
5'-3' double-stranded DNA exonuclease
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan][category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes][category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan][category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.7|Mismatch repair (MMR)][SW|Categories] containing this gene/protein
[SW|cell division], [SW|cell wall synthesis], [SW|DNA repair/ recombination]Gene
Coordinates on the chromosome (coding sequence)
4,148,851 -> 4,149,645
Phenotypes of a mutant
the mutant divides over unsegregated chromosomes more frequently than wild-type cells, and this phenotype is exacerbated when DNA replication is inhibited [Pubmed|21169496]increased sensitivity to a narrow spectrum of cephalosporin antibiotics [Pubmed|21169496]disruption of the corresponding gene in B. anthracis results in a mutator phenotype [Pubmed|21205011]The protein
Catalyzed reaction/ biological activity
5'-3' exonuclease activity at the 5' termini and at nicks of double-stranded DNA [Pubmed|23491602]the enzymatic activity of WalJ directly or indirectly affects cell wall metabolism and is required for accurate coordination of [SW|cell division] with DNA replication [Pubmed|21169496]Protein family
predicted member of the metallo-β-lactamase superfamily[SW|Cofactors]
Mn(II) [Pubmed|23491602]Expression and Regulation
Operon
''[protein|search|walR]-[protein|search|walK]-[protein|search|yycH]-[protein|search|yycI]-[protein|search|walJ]-[protein|search|htrC]'' [Pubmed|9829949]Regulation
expressed during vegetative growth, repressed during stationary phase [Pubmed|9829949]Biological materials
Mutant
MGNA-B824 (yycJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1823 NBRP B. subtilis, Japan]1A1044 ( ''walJ''::''spec''), [Pubmed|21169496], available at [BGhttp://bgsc.org/getdetail.php?bgscid=1A1044]BKE40370 (''walJ''::''erm trpC2''), available at [http://bgsc.org]References
Reviews
Original publications
9829949,21169496,21205011,23491602 
