cotH [2020-06-29 09:58:04]

protein kinase, required for the assembly of several outer coat proteins

Locus: BSU_36060

Isoelectric point: 5.95

Molecular weight: 42.65 kDa

Protein length: 362 aa

Gene length: 1089 bp

Function: protection of CotU and CotC in the mother cell

Product: protein kinase

Essential: no

Synonyms: ywrH

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Sporulation → Sporulation proteins → Spore coat proteins → Not yet assigned

Gene

Coordinates: 3,716,009 → 3,717,097

Phenotypes of a mutant

• overexpression of cotH suppresses the phenotypes of a cotE mutant PubMed
• reduced germination efficiency PubMed

The protein

Catalyzed reaction/ biological activity

• autophosphorylation with ATP and subsequent phosphorylation of CotB and CotG on serine residues PubMed

Protein family

• cotH family (with YisJ, according to UniProt)

Cofactors

• Mn²⁺ PubMed

Structure

• 5JD9 PubMed

Localization

• inner spore coat

Expression and Regulation

Operons

• Genes: cotH-cotB-ywrJ
  Description: PubMed

  Sigma factors

  • SigK: sigma factor, PubMed, in SigK regulon
  • SigE: sigma factor, PubMed, in SigE regulon

  Regulatory mechanism

  • GerR: activation, PubMed, in GerR regulon
  • GerE: repression, PubMed, in GerE regulon
  • SpoIIID: activation, PubMed, in SpoIIID regulon

  Regulation

  • expressed late during sporulation in the mother cell (SigE, SigK, GerE, SpoIIID) PubMed

Biological materials

Mutant

• BKE36060 (ΔcotH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCATAATCCTCCTTACA, downstream forward: _UP4_TGAATGCGTGAAAATGGGTA
• BKK36060 (ΔcotH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCATAATCCTCCTTACA, downstream forward: _UP4_TGAATGCGTGAAAATGGGTA

References

Reviews

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Original Publications

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