UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase, conversion of lipid I to lipid II
function
peptidoglycan precursor biosynthesis
product
UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan][category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan][category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins][SW|Categories] containing this gene/protein
[SW|cell wall synthesis], [SW|biosynthesis of cell wall components], [SW|sporulation proteins], [SW|essential genes], [SW|membrane proteins]This gene is a member of the following [SW|regulons]
[SW|SigE regulon], [SW|SpoIIID regulon]Gene
Coordinates on the chromosome (coding sequence)
1,591,540 -> 1,592,631
Phenotypes of a mutant
essential [Pubmed|12682299]The protein
Catalyzed reaction/ biological activity
converts lipid I to lipid II by attaching GlcNAc to the bactoprenol carrier-conjugated UDP-N-acetylmuramic acid (UDP-MurNAc) pentapeptide moleculeUDP-N-acetylglucosamine Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol (according to Swiss-Prot)Protein family
MurG subfamily (according to Swiss-Prot)Structure
[PDB|1F0K] (from ''Escherichia coli'', 30% identity, 52% similarity) [Pubmed|10892798][SW|Localization]
peripheral membrane protein [Pubmed|24706874]forms banded patterns along the length of the cell, this pattern formation depends on [SW|MreB] [Pubmed|17880425]Expression and Regulation
Operon
''[protein|search|murE]-[protein|search|mraY]-[protein|search|murD]-[SW|spoVE]-[protein|search|murG]-[protein|search|murB]-[SW|divIB]-[protein|search|ylxW]-[protein|search|ylxX]-[protein|search|sbp]'' [Pubmed|1391053]''[SW|spoVE]-[protein|search|murG]-[protein|search|murB]-[SW|divIB]-[protein|search|ylxW]-[protein|search|ylxX]-[protein|search|sbp]'' [Pubmed|1391053]''[protein|search|murG]-[protein|search|murB]-[SW|divIB]-[protein|search|ylxW]-[protein|search|ylxX]-[protein|search|sbp]'' [Pubmed|18179421][SW|Sigma factor]
''[SW|spoVE]'': [protein|search|SigE] [Pubmed|8320223]within ''[protein|search|murG]'': [protein|search|SigM] [Pubmed|18179421]Regulation
expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|1391053]Regulatory mechanism
[SW|SpoIIID]: transcription repression [Pubmed|15383836]Biological materials
FLAG-tag construct
GP2002 ''murG-3xFLAG ermC'' (based on [SW|pGP1087]), available in [SW|Jörg Stülke]'s labReferences
22092867,18063720,18081839,1391053,8320223,15383836,18179421,10790316,24706874,26278972,17880425,20233306 
