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murG
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murG

UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase, conversion of lipid I to lipid II
Locus
BSU_15220
Isoelectric point
9.57
Molecular weight
39.82 kDa
Protein length
363 aa Sequence Blast
Gene length
1092 bp Sequence Blast
Function
peptidoglycan precursor biosynthesis
Product
UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
Essential
yes
E.C.
2.4.1.227
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,591,540 → 1,592,631

Phenotypes of a mutant

  • essential PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • converts lipid I to lipid II by attaching GlcNAc to the bactoprenol carrier-conjugated UDP-N-acetylmuramic acid (UDP-MurNAc) pentapeptide molecule
  • Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol + UDP-N-acetyl-α-D-glucosamine --> β-D-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol + H+ + UDP (according to UniProt)
  • Protein family

  • glycosyltransferase 28 family (with UgtP and YkoN, according to UniProt)
  • Structure

  • 1F0K (from Escherichia coli, 30% identity, 52% similarity) PubMed
  • Localization

  • peripheral membrane protein PubMed
  • in the presence of daptomycin, MurG delocalizes from the membrane to the cytoplasm PubMed
  • forms banded patterns along the length of the cell, this pattern formation depends on MreB PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigM: sigma factor, (promoter within MurG) PubMed, in SigM regulon
  • SigA: sigma factor, PubMed, in SigA regulon
  • SigE: sigma factor, PubMed, in SigE regulon
  • Regulatory mechanism

  • SpoIIID: repression, PubMed, in SpoIIID regulon
  • Regulation

  • expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell (SigE) PubMed
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    Description

    Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon
  • Regulatory mechanism

  • stringent response: negative regulation, in stringent response
  • SpoIIID: repression, PubMed, in SpoIIID regulon
  • Regulation

  • expressed during vegatative growth PubMed
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    Description

    Regulation

  • expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell (SigE) PubMed
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    Biological materials

    FLAG-tag construct

  • GP2002 murG-3xFLAG ermC (based on pGP1087), available in Jörg Stülke's lab
  • References

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